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验证数据展示

  • WB analysis using 18138-1-AP

    WB analysis using 18138-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18138-1-AP (ABCD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 18138-1-AP

    WB analysis of HeLa using 18138-1-AP

    WB result of ABCD1 antibody (18138-1-AP, 1:600) with si-Control and si-ABCD1 transfected HeLa cells..

  • IP experiment of HeLa using 18138-1-AP

    IP experiment of HeLa using 18138-1-AP

    IP result of anti-ABCD1 (IP:18138-1-AP, 4ug; Detection:18138-1-AP 1:700) with HeLa cells lysate 3000 ug.

  • FC experiment of HeLa using 18138-1-AP

    FC experiment of HeLa using 18138-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ABCD1 (18138-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • WB analysis of HepG2 using 18138-1-AP

    WB analysis of HepG2 using 18138-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18138-1-AP (ABCD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 18138-1-AP

    IHC staining of human lung cancer using 18138-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18138-1-AP (ABCD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ABCD1 antibody included
  • IHC staining of human ovary cancer using 18138-1-AP

    IHC staining of human ovary cancer using 18138-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18138-1-AP (ABCD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ABCD1 antibody included
  • IHC staining of human ovary cancer using 18138-1-AP

    IHC staining of human ovary cancer using 18138-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18138-1-AP (ABCD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ABCD1 antibody included
  • IF Staining of HeLa using 18138-1-AP

    IF Staining of HeLa using 18138-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ABCD1 antibody (18138-1-AP) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Featured Product
  • KD/KO Validated

ABCD1 Polyclonal antibody

ABCD1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 18138-1-AP

产品说明书

引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

别名

ABCD 1, ALD, ATP binding cassette sub family D member 1, ATP-binding cassette sub-family D member 1, EC:3.1.2.-

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ABCD1 Ready-To-Use IHC Kit. ABCD1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18138-1-AP targets ABCD1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag12757

Product name: Recombinant human ABCD1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 358-745 aa of BC025358

Sequence: SESDAEAVKKAALEKKEEELVSERTEAFTIARNLLTAAADAIERIMSSYKEVTELAGYTARVHEMFQVFEDVQRCHFKRPRELEDAQAGSGTIGRSGVRVEGPLKIRGQVVDVEQGIICENIPIVTPSGEVVVASLNIRVEEGMHLLITGPNGCGKSSLFRILGGLWPTYGGVLYKPPPQRMFYIPQRPYMSVGSLRDQVIYPDSVEDMQRKGYSEQDLEAILDVVHLHHILQREGGWEAMCDWKDVLSGGEKQRIGMARMFYHRPKYALLDECTSAVSIDVEGKIFQAAKDAGIALLSITHRPSLWKYHTHLLQFDGEGGWKFEKLDSAARLSLTEEKQRLEQQLAGIPKMQRRLQELCQILGEAVAPAHVPAPSPQGPGGLQGAST

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATP-binding cassette, sub-family D (ALD), member 1
别名 ABCD 1, ALD, ATP binding cassette sub family D member 1, ATP-binding cassette sub-family D member 1, EC:3.1.2.-
计算分子量 745 aa, 83 kDa
观测分子量 75 kDa
GenBank蛋白编号BC025358
基因名称 ABCD1
Gene ID (NCBI) 215
RRIDAB_2273333
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP33897
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABCD1 (also known as ALDP) is a member of the ATP-binding cassette (ABC) transporter superfamily which functions as transporter for a wide variety of substrates. It localizes to the peroxisomal membrane. The exact function is not clear so far. Various mutations of ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex.

实验方案

Product Specific Protocols
FC protocol for ABCD1 antibody 18138-1-APDownload protocol
IF protocol for ABCD1 antibody 18138-1-APDownload protocol
IHC protocol for ABCD1 antibody 18138-1-APDownload protocol
IP protocol for ABCD1 antibody 18138-1-APDownload protocol
WB protocol for ABCD1 antibody 18138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Signal Transduct Target Ther

Structural insights into substrate recognition and translocation of human peroxisomal ABC transporter ALDP

Authors - Chao Xiong
View Article
humanWB

Leukemia

Targeting ABCD1-ACOX1-MET/IGF1R axis suppresses multiple myeloma

Authors - Zhannan Han
View Article
humanWB

EMBO J

Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.

Authors - Tatsuo Miyamoto
  • KO Validated
View Article
humanWB

Int Endod J

Mitochondrial Function Is Required to Preserve Viability of Stem Cells From the Apical Papilla Following Porphyromonas endodontalis Infection.

Authors - S Lei
View Article
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