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验证数据展示

  • WB analysis of MCF-7 using 27158-1-AP (same clone as 27158-1-PBS)

    WB analysis of MCF-7 using 27158-1-AP (same clone as 27158-1-PBS)

    WB result of ABCB8 antibody (27158-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ABCB8 transfected MCF-7 cells. This data was developed using the same antibody clone with 27158-1-PBS in a different storage buffer formulation.

  • WB analysis using 27158-1-AP (same clone as 27158-1-PBS)

    WB analysis using 27158-1-AP (same clone as 27158-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27158-1-AP (ABCB8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27158-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 27158-1-AP (same clone as 27158-1-PBS)

    IHC staining of mouse liver using 27158-1-AP (same clone as 27158-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27158-1-AP (ABCB8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27158-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 27158-1-AP (same clone as 27158-1-PBS)

    IHC staining of mouse liver using 27158-1-AP (same clone as 27158-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27158-1-AP (ABCB8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27158-1-PBS in a different storage buffer formulation.

  • IF Staining of H9C2 using 27158-1-AP (same clone as 27158-1-PBS)

    IF Staining of H9C2 using 27158-1-AP (same clone as 27158-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using ABCB8 antibody (27158-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27158-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ABCB8 Polyclonal antibody, PBS Only

ABCB8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27158-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

ABCB 8, ATP Binding Cassette Subfamily B Member 8, ATP-binding cassette sub-family B member 8, mitochondrial, M ABC1, MABC1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

27158-1-PBS targets ABCB8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag25935

Product name: Recombinant human ABCB8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 403-596 aa of BC141814

Sequence: TVQRSMANLSVLFGQVVRGLSAGARVFEYMALNPCIPLSGGCCVPKEQLRGSVTFQNVCFSYPCRPGFEVLKDFTLTLPPGKIVALVGQSGGGKTTVASLLERFYDPTAGVVMLDGRDLRTLDPSWLRGQVVGFISQEPVLFGTTIMENIRFGKLEASDEEVYTAAREANAHEFITSFPEGYNTVVGERGTTLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATP-binding cassette, sub-family B (MDR/TAP), member 8
别名 ABCB 8, ATP Binding Cassette Subfamily B Member 8, ATP-binding cassette sub-family B member 8, mitochondrial, M ABC1, MABC1
计算分子量 735 aa, 80 kDa
观测分子量60-65 kDa
GenBank蛋白编号BC141814
基因名称 ABCB8
Gene ID (NCBI) 11194
RRIDAB_3742217
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NUT2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ABCB8 (ATP-binding cassette sub-family B member 8) is a protein located in the mitochondrial inner membrane and belongs to the ATP-binding cassette (ABC) transporter superfamily. It plays a crucial role in maintaining mitochondrial iron homeostasis and the maturation of cellular iron-sulfur cluster proteins. ABCB8 is essential for normal cardiac function by promoting the export of iron from mitochondria. Additionally, ABCB8 forms a complex with the mitochondrial KATP channel, protecting cells from oxidative stress damage. The loss of function or abnormal expression of ABCB8 is associated with various diseases, including cardiomyopathy and iron metabolism disorders.

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