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验证数据展示

  • WB analysis of MCF-7 using 27158-1-AP

    WB analysis of MCF-7 using 27158-1-AP

    WB result of ABCB8 antibody (27158-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ABCB8 transfected MCF-7 cells.

  • WB analysis using 27158-1-AP

    WB analysis using 27158-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27158-1-AP (ABCB8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 27158-1-AP

    IHC staining of mouse liver using 27158-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27158-1-AP (ABCB8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 27158-1-AP

    IHC staining of mouse liver using 27158-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27158-1-AP (ABCB8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of H9C2 using 27158-1-AP

    IF Staining of H9C2 using 27158-1-AP

    Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using ABCB8 antibody (27158-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

ABCB8 Polyclonal antibody

ABCB8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27158-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

ABCB 8, ATP Binding Cassette Subfamily B Member 8, ATP-binding cassette sub-family B member 8, mitochondrial, M ABC1, MABC1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, MCF-7 cells, MDA-MB-231 cells, mouse liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inH9C2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27158-1-AP targets ABCB8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ABCB8 fusion protein Ag25935 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATP-binding cassette, sub-family B (MDR/TAP), member 8
别名 ABCB 8, ATP Binding Cassette Subfamily B Member 8, ATP-binding cassette sub-family B member 8, mitochondrial, M ABC1, MABC1
计算分子量 735 aa, 80 kDa
观测分子量60-65 kDa
GenBank蛋白编号BC141814
基因名称 ABCB8
Gene ID (NCBI) 11194
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NUT2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABCB8 (ATP-binding cassette sub-family B member 8) is a protein located in the mitochondrial inner membrane and belongs to the ATP-binding cassette (ABC) transporter superfamily. It plays a crucial role in maintaining mitochondrial iron homeostasis and the maturation of cellular iron-sulfur cluster proteins. ABCB8 is essential for normal cardiac function by promoting the export of iron from mitochondria. Additionally, ABCB8 forms a complex with the mitochondrial KATP channel, protecting cells from oxidative stress damage. The loss of function or abnormal expression of ABCB8 is associated with various diseases, including cardiomyopathy and iron metabolism disorders.

实验方案

Product Specific Protocols
WB protocol for ABCB8 antibody 27158-1-APDownload protocol
IHC protocol for ABCB8 antibody 27158-1-APDownload protocol
IF protocol for ABCB8 antibody 27158-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols