验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, Neuro-2a cells, HepG2 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 2 publications below |
产品信息
67868-1-Ig targets AATF in WB, IHC, ELISA, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, ELISA, FC (Intra) Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | Human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag0195 Product name: Recombinant human AATF protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 7-212 aa of BC000591 Sequence: LALQLEQLLNPRPSEADPEADPEEATAARVIDRFDEGEDGEGDFLVVGSIRKLASASLLDTDKRYCGKTTSRKAWNEDHWEQTLPGSSDEEISDEEGSGDEDSEGLGLEEYDEDDLGAAEEQECGDHRESKKSRSHSAKTPGFSVQSISDFEKFTKGMDDLGSSEEEEDEESGMEEGDDAEDSQGESEEDRAGDRNSEDDGVVMTF 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | apoptosis antagonizing transcription factor |
| 别名 | Protein AATF, HSPC277, DED, CHE1, CHE 1 |
| 计算分子量 | 63 kDa |
| 观测分子量 | 80-90 kDa |
| GenBank蛋白编号 | BC000591 |
| 基因名称 | AATF |
| Gene ID (NCBI) | 26574 |
| RRID | AB_2918626 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9NY61 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AATF antibody 67868-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Onco Targets Ther AATF is Overexpressed in Human Head and Neck Squamous Cell Carcinoma and Regulates STAT3/Survivin Signaling | ||
Onco Targets Ther AATF is Overexpressed in Human Bladder Cancer and Regulates Chemo-Sensitivity Through Survivin. |
