验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue, human brain tissue, human testis tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
55163-1-AP targets PPP3CC-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein phosphatase 3 (formerly 2B), catalytic subunit, gamma isoform |
| 别名 | PPP3CC, CNA3, CAM-PRP catalytic subunit, CAM PRP catalytic subunit, CALNA3 |
| 计算分子量 | 58 kDa |
| 观测分子量 | 58 kDa |
| GenBank蛋白编号 | NM_005605 |
| 基因名称 | PPP3CC |
| Gene ID (NCBI) | 5533 |
| RRID | AB_10860906 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P48454 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PPP3CC, also named as CALNA3 and CNA3, belongs to the PPP phosphatase family and PP-2B subfamily. It is a calcium-dependent, calmodulin-stimulated protein phosphatase. It may have a role in the calmodulin activation of calcineurin. This antibody is specific to PPP3CC.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for PPP3CC-specific antibody 55163-1-AP | Download protocol |
| IHC protocol for PPP3CC-specific antibody 55163-1-AP | Download protocol |
| WB protocol for PPP3CC-specific antibody 55163-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Acta Biomater Substrate stiffness regulates differentiation of induced pluripotent stem cells into heart valve endothelial cells. | ||
BMC Pregnancy Childbirth Core biomarkers analysis benefit for diagnosis on human intrahepatic cholestasis of pregnancy |
