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验证数据展示

  • FC experiment of human PBMCs using SF98054-1-RR

    FC experiment of human PBMCs using SF98054-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD5 Rabbit scFv Antibody (SF98054-1-RR, Clone: 240428A12) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE Anti-GGGGS Linker Rabbit Recombinant Antibody. Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed.

  • SDS-PAGE of SF98054-1-RR

    SDS-PAGE of SF98054-1-RR

    Purity of Recombinant ScFv antibody(Cat No.:SF98054-1-RR) was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and condition and stained using Coomassie blue.

  • Affinity and Kinetic Characterization of SF98054-1-RR

    Affinity and Kinetic Characterization of SF98054-1-RR

    Biolayer interferometry (BLl) kinetic assays of SF98054-1-RR against Human CD5 were performed. The affinity constant is below 1 pM.

  • Affinity and Kinetic Characterization of SF98054-1-RR

    Affinity and Kinetic Characterization of SF98054-1-RR

    Biolayer interferometry (BLl) kinetic assays of SF98054-1-RR against Human CD4 were performed. The affinity constant is 0.416 nM.

Anti-Human CD5 Rabbit scFv Antibody

CD5 Recombinant Antibody for FC
Cat No. SF98054-1-RR

产品说明书

CloneNo. 240428A12

宿主/类型

Rabbit / scFv

种属反应性

human

应用

FC

别名

CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, Lymphocyte antigen T1/Leu-1, T1

缓冲液配方:  HEPES, EDTA, CHAPS, Sucrose, Proclin300
HEPESPBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy® CD5 Ready-To-Use IHC Kit. CD5 primary antibody included.

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

SF98054-1-RR targets CD5 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

CatNo: Eg1119

Product name: Recombinant Human CD5 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 25-372 aa of NM_014207.4

Sequence: RLSWYDPDFQARLTRSNSKCQGQLEVYLKDGWHMVCSQSWGRSSKQWEDPSQASKVCQRLNCGVPLSLGPFLVTYTPQSSIICYGQLGSFSNCSHSRNDMCHSLGLTCLEPQKTTPPTTRPPPTTTPEPTAPPRLQLVAQSGGQHCAGVVEFYSGSLGGTISYEAQDKTQDLENFLCNNLQCGSFLKHLPETEAGRAQDPGEPREHQPLPIQWKIQNSSCTSLEHCFRKIKPQKSGRVLALLCSGFQPKVQSRLVGGSSICEGTVEVRQGAQWAALCDSSSARSSLRWEEVCREQQCGSVNSYRVLDAGDPTSRGLFCPHQKLSQCHELWERNSYCKKVFVTCQDPNP

种属同源性预测
宿主/类型 Rabbit / scFv
抗体类别 Recombinant
产品类型 Single-chain variable fragment antibody (scFv)
全称 CD5 molecule
别名 CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, Lymphocyte antigen T1/Leu-1, T1
计算分子量55 kDa
GenBank蛋白编号NM_014207.4
基因名称 CD5
Gene ID (NCBI) 921
ENSEMBL Gene IDENSG00000110448
RRIDAB_3750145
偶联类型 Unconjugated
来源Anti-Human CD5 scFv from clone 240428A12, consisting of VH-GGGGS linker-VL (N- to C-terminus), with a His-C tag at the C-terminus.
形式Liquid
纯化方式Affinity purification
储存缓冲液 50mM HEPES, 150mM NaCl, 5mM EDTA, 0.1% CHAPS, 10% Sucrose and 0.1% Proclin300, pH 7.3.
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

实验方案

Product Specific Protocols
FC protocol for CD5 antibody SF98054-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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