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验证数据展示

  • FC experiment of human PBMCs using SF98042-1-RR

    FC experiment of human PBMCs using SF98042-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug CD4 Recombinant Antibody (SF98042-1-RR, Clone: 240427E12) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE Anti-GGGGS Linker Rabbit Recombinant Antibody (PE-98262, Clone: 242306C8). Cells were then stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed. Lymphocytes were gated.

  • SDS-PAGE of SF98042-1-RR

    SDS-PAGE of SF98042-1-RR

    Purity of Recombinant ScFv antibody(Cat No.:SF98042-1-RR) was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and condition and stained using Coomassie blue.

  • Affinity and Kinetic Characterization of SF98042-1-RR

    Affinity and Kinetic Characterization of SF98042-1-RR

    Biolayer interferometry (BLl) kinetic assays of SF98042-1-RR against Human CD4 were performed. The affinity constant is 0.824 nM.

Anti-Human CD4 Rabbit scFv Antibody

CD4 Recombinant Antibody for FC
Cat No. SF98042-1-RR

产品说明书

CloneNo. 240427E12

宿主/类型

Rabbit / scFv

种属反应性

human, monkey, non-human primates

应用

FC

别名

CD4 molecule, CD4mut, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

缓冲液配方:  HEPES, EDTA, CHAPS, Sucrose, Proclin300
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

SF98042-1-RR targets CD4 in FC applications and shows reactivity with human, monkey, non-human primates samples.

经测试应用 FC Application Description
经测试反应性 human, monkey, non-human primates
免疫原

CatNo: Eg0333

Product name: Recombinant Human CD4 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 26-396 aa of BC025782

Sequence: KKVVLGKKGDTVELTCTASQKKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDSDTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNIQGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFSFPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLHLTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLMLSLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQP

种属同源性预测
宿主/类型 Rabbit / scFv
抗体类别 Recombinant
产品类型 Single-chain variable fragment antibody (scFv)
全称 CD4 molecule
别名 CD4 molecule, CD4mut, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
计算分子量 55 kDa
GenBank蛋白编号BC025782
基因名称 CD4
Gene ID (NCBI) 920
ENSEMBL Gene IDENSG00000010610
RRIDAB_3750144
偶联类型 Unconjugated
来源Anti-Human CD4 scFv from clone 240427E12, consisting of VH-GGGGS linker-VL (N- to C-terminus), with a His-C tag at the C-terminus.
形式Liquid
纯化方式Affinity purification
储存缓冲液 50mM HEPES, 150mM NaCl, 5mM EDTA, 0.1% CHAPS, 10% Sucrose and 0.1% Proclin 300, pH 7.3.
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, the majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

实验方案

Product Specific Protocols
FC protocol for CD4 antibody SF98042-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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