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验证数据展示

  • FC experiment of human PBMCs using 98599-1-MR

    FC experiment of human PBMCs using 98599-1-MR

    1x10^6 human PBMCs were unstained (left, blue in histogram) or surface stained with 0.25 ug Mouse IgG2a, κ Isotype Control RecAb (98599-1-MR, Clone:252252A1) (middle, red in histogram) or 0.25 ug Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (65569-1-MR, Clone: OKT3) (right, green in histogram) and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of mouse bone marrow cells using 98599-1-MR

    FC experiment of mouse bone marrow cells using 98599-1-MR

    1x10^6 mouse bone marrow cells were unstained (left, blue in histogram) or surface stained with 0.25 ug Mouse IgG2a, κ Isotype Control RecAb (98599-1-MR, Clone:252252A1) (middle, red in histogram) or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (right, green in histogram) and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

Mouse IgG2a, κ Isotype Control Recombinant Antibody

IgG2a, κ Isotype Control Uni-rAb® Recombinant Antibody for FC
Cat No. 98599-1-MR

产品说明书

CloneNo. 252252A1

宿主/亚型

Mouse / IgG2a

种属反应性

n/a

应用

FC

别名

IgG2a Isotype Control, Mouse IgG2a, IgG2a

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inhuman PBMCs, THP-1 cells, HL-60 cells, mouse bone marrow cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98599-1-MR targets IgG2a, κ Isotype Control in FC applications and shows reactivity with n/a samples.

经测试应用 FC Application Description
经测试反应性 n/a
免疫原

unknow

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 IgG2a, κ Isotype Control
别名 IgG2a Isotype Control, Mouse IgG2a, IgG2a
基因名称
Gene ID (NCBI)
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

This product is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

实验方案

Product Specific Protocols
FC protocol for IgG2a, κ Isotype Control antibody 98599-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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