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验证数据展示

  • FC experiment of Transfected HEK-293T using 98519-1-RR

    FC experiment of Transfected HEK-293T using 98519-1-RR

    1x10^6 HEK-293T cells or CCL20/MIP-3 alpha transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse CCL20/MIP-3 alpha Rabbit RecAb (98519-1-RR, Clone: 250239E12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of Transfected HEK-293T using 98519-1-RR

    FC experiment of Transfected HEK-293T using 98519-1-RR

    1x10^6 HEK-293T cells or CCL20/MIP-3 alpha-transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse CCL20/MIP-3 alpha Rabbit RecAb (98519-1-RR, Clone: 250239E12) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Anti-Mouse CCL20/MIP-3 alpha Rabbit Recombinant Antibody

CCL20/MIP-3 alpha Recombinant Antibody for FC (Intra)
Cat No. 98519-1-RR

产品说明书

CloneNo. 250239E12

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC (Intra)

别名

Beta-chemokine exodus-1, CC chemokine LARC, CC chemokine ST38, C-C motif chemokine 20, CCL 20

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inTransfected HEK-293T cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98519-1-RR targets CCL20/MIP-3 alpha in FC (Intra) applications and shows reactivity with mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 mouse
免疫原 CCL20/MIP-3 alpha fusion protein Eg3311 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chemokine (C-C motif) ligand 20
别名 Beta-chemokine exodus-1, CC chemokine LARC, CC chemokine ST38, C-C motif chemokine 20, CCL 20
计算分子量11KD
GenBank蛋白编号NM_001159738.1
基因名称 Ccl20
Gene ID (NCBI) 20297
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ642U4
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

CCL20, also known as macrophage infiltrating factor protein 3α, is a C-C chemokine that specifically binds to the receptor CCR6. CCL20 is produced in various tissues and by a wide range of immune cells, including neutrophils, natural killer cells, T-helper type 17 (Th17) cells, B cells, and various antigen-presenting cells, such as dendritic cells (DCs), Langerhans cells, and macrophages. Increased expression of CCL20 is likely involved in the increased recruitment of dendritic cells observed in fibroinflammatory diseases such as chronic obstructive pulmonary disease (COPD). CCL20 expression is increased by the proinflammatory cytokine IL-1 beta.

实验方案

Product Specific Protocols
FC protocol for CCL20/MIP-3 alpha antibody 98519-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols