验证数据展示
经过测试的应用
| Positive FC detected in | mouse peripheral blood platelets |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
98516-2-RR targets JAM-A/CD321 in FC applications and shows reactivity with mouse samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg3117 Product name: Recombinant Mouse JAM-A/Junctional Adhesion Molecule A protein (rFc Tag) (HPLC verified) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 27-238 aa of NM_172647.2 Sequence: KGSVYTAQSDVQVPENESIKLTCTYSGFSSPRVEWKFVQGSTTALVCYNSQITAPYADRVTFSSSGITFSSVTRKDNGEYTCMVSEEGGQNYGEVSIHLTVLVPPSKPTISVPSSVTIGNRAVLTCSEHDGSPPSEYSWFKDGISMLTADAKKTRAFMNSSFTIDPKSGDLIFDPVTAFDSGEYYCQAQNGYGTAMRSEAAHMDAVELNVGG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | F11 receptor |
| 别名 | CD321, F11r, Jam1, JAM-1, JAM-A |
| 计算分子量 | 32 kDa |
| GenBank蛋白编号 | NM_172647.2 |
| 基因名称 | F11r |
| Gene ID (NCBI) | 16456 |
| RRID | AB_3746145 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O88792 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2 - 8°C. Stable for one year after shipment. |
背景介绍
Junctional adhesion molecule A (JAM-A), also known as CD321 or F11 receptor (F11R), is a transmembrane protein that belongs to the immunoglobulin superfamily of cell adhesion molecules. JAM-A is present in epithelial cells, endothelial cells, leukocytes, and blood platelets. JAM-A participates in regulating various biological processes, as diverse as paracellular permeability, tight junction formation and maintenance, leukocyte transendothelial migration, epithelial-to-mesenchymal transition, angiogenesis, reovirus binding, and platelet activation.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for JAM-A/CD321 antibody 98516-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
