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验证数据展示

  • FC experiment of Transfected CHO using 98512-2-RR

    FC experiment of Transfected CHO using 98512-2-RR

    1x10^6 CHO cells or IL-20RA-transfected CHO cells were surface stained with 0.25 ug Anti-Human IL-20RA Rabbit RecAb (98512-2-RR, Clone: 250321B10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • FC experiment of Transfected CHO using 98512-2-RR

    FC experiment of Transfected CHO using 98512-2-RR

    1x10^6 CHO cells or IL-20RA-transfected CHO cells were surface stained with 0.25 ug Anti-Human IL-20RA Rabbit RecAb (98512-2-RR, Clone: 250321B10) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • FC experiment of T47D using 98512-2-RR

    FC experiment of T47D using 98512-2-RR

    1x10^6 T-47D cells were surface stained with 0.25 ug Anti-Human IL-20RA Rabbit RecAb (98512-2-RR, Clone: 250321B10) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

Anti-Human IL-20RA Rabbit Recombinant Antibody

IL-20RA Uni-rAb® Recombinant Antibody for FC
Cat No. 98512-2-RR

产品说明书

CloneNo. 250321B10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

IL20RA, CRF2 8, IL 20 receptor subunit alpha, IL 20R alpha, IL 20R1

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inTransfected CHO cells, T-47D cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98512-2-RR targets IL-20RA in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

CatNo: Eg2780

Product name: Recombinant Human IL-20RA protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 30-250 aa of NM_014432

Sequence: VPCVSGGLPKPANITFLSINMKNVLQWTPPEGLQGVKVTYTVQYFIYGQKKWLNKSECRNINRTYCDLSAETSDYEHQYYAKVKAIWGTKCSKWAESGRFYPFLETQIGPPEVALTTDEKSISVVLTAPEKWKRNPEDLPVSMQQIYSNLKYNVSVLNTKSNRTWSQCVTNHTLVLTWLEPNTLYCVHVESFVPGPPRRAQPSEKQCARTLKDQSSEFKAK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 20 receptor, alpha
别名 IL20RA, CRF2 8, IL 20 receptor subunit alpha, IL 20R alpha, IL 20R1
计算分子量62 kDa
GenBank蛋白编号NM_014432
基因名称 IL20RA
Gene ID (NCBI) 53832
RRIDAB_3746142
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UHF4-1
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

Interleukin 20 receptor subunit alpha (IL-20RA) belongs to the type II cytokine receptor family. Upon binding to its ligands, such as interleukin (IL)-19, IL-20, and IL-24, IL-20RA can form a functional heterodimeric receptor with IL-20RB. IL-26, another IL-20RA ligand, requires IL-20RA and interleukin 10 receptor subunit beta (IL-10RB) for signaling (PMID: 25421700; 11163236; 15178681).

实验方案

Product Specific Protocols
FC protocol for IL-20RA antibody 98512-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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