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验证数据展示

  • FC experiment of human peripheral blood leukocytes using 98499-1-RR

    FC experiment of human peripheral blood leukocytes using 98499-1-RR

    1x10^6 human peripheral blood leukocytes were intracellularly stained with 0.25 ug Anti-Human Neutrophil Elastase Rabbit RecAb (98499-1-RR, Clone: 250346G5) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • FC experiment of U-937 using 98499-1-RR

    FC experiment of U-937 using 98499-1-RR

    1x10^6 U-937 cells (left) or Jurkat cells (right) were intracellularly stained with 0.25 ug Anti-Human Neutrophil Elastase Rabbit RecAb (98499-1-RR, Clone: 250346G5) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Anti-Human Neutrophil Elastase Rabbit Recombinant Antibody

Neutrophil Elastase Uni-rAb® Recombinant Antibody for FC (Intra)
Cat No. 98499-1-RR

产品说明书

CloneNo. 250346G5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

ELA2, ELANE, EC:3.4.21.37, Elastase 2, HLE

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inhuman peripheral blood leukocytes, U-937 cells
Planning an IHC experiment? We recommend our IHCeasy ELANE/ELA2 Ready-To-Use IHC Kit. ELANE/ELA2 primary antibody included.

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98499-1-RR targets Neutrophil Elastase in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Eg3192

Product name: Recombinant Human Neutrophil Elastase protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 28-267 aa of NM_001972.4

Sequence: SEIVGGRRARPHAWPFMVSLQLRGGHFCGATLIAPNFVMSAAHCVANVNVRAVRVVLGAHNLSRREPTRQVFAVQRIFENGYDPVNLLNDIVILQLNGSATINANVQVAQLPAQGRRLGNGVQCLAMGWGLLGRNRGIASVLQELNVTVVTSLCRRSNVCTLVRGRQAGVCFGDSGSPLVCNGLIHGIASFVRGGCASGLYPDAFAPVAQFVNWIDSIIQRSEDNPCPHPRDPDPASRTH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 elastase 2, neutrophil
别名 ELA2, ELANE, EC:3.4.21.37, Elastase 2, HLE
计算分子量29KD
GenBank蛋白编号NM_001972.4
基因名称 ELA2
Gene ID (NCBI) 1991
RRIDAB_3746129
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP08246
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

ELA2(Neutrophil elastase) is also named as ELAL, NE, HLE, HNE, PMN-E, SERP1, GE, ELANE and belongs to the peptidase S1 family. It is a 33-kDa enzyme with several isoforms that differ in their extent of glycosylation (PMID:12223222). Human neutrophil elastase (HNE) is a serine protease with potent proteolytic activity, which is reported to cause mucin secretion (degranulation) (PMID:12169572). It may contribute to the directed migration of leukocytes into flamed postcapillary venules in addition to contributing to the process of tissue emigration (PMID:9683424). The full length protein has a signal peptide,propeptide and three glycosylation sites.

实验方案

Product Specific Protocols
FC protocol for Neutrophil Elastase antibody 98499-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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