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验证数据展示

  • FC experiment of human PBMCs using 98477-1-RR

    FC experiment of human PBMCs using 98477-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human LILRA5/CD85f Rabbit RecAb (98477-1-RR, Clone:250298G11) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD14 Rabbit Recombinant Antibody (APC-98040, Clone: 230332D7). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

  • FC experiment of human PBMCs using 98477-1-RR

    FC experiment of human PBMCs using 98477-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human LILRA5/CD85f Rabbit RecAb (98477-1-RR, Clone: 250298G11) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

Anti-Human LILRA5/CD85f Rabbit Recombinant Antibody

LILRA5/CD85f Uni-rAbTM Recombinant Antibody for FC
Cat No. 98477-1-RR

产品说明书

CloneNo. 250298G11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

LILRA5, CD85, CD85 antigen-like family member F, CD85f, ILT 11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98477-1-RR targets LILRA5/CD85f in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 LILRA5/CD85f fusion protein Eg2737 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 5
别名 LILRA5, CD85, CD85 antigen-like family member F, CD85f, ILT 11
计算分子量33 kDa
GenBank蛋白编号NM_021250.4
基因名称 LILRA5
Gene ID (NCBI) 353514
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDA6NI73-1
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

LILRA5, also named as CD85f, ILT11, LILRB7 or LIR9, is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is a group of immune inhibitory and stimulatory receptors (PMID: 15304001). There are two membrane-bound and two soluble forms of LILRA5. The transmembrane LILRA5 contains a short cytoplasmic domain and a charged arginine residue within the transmembrane region (PMID: 19009525). It is mostly expressed in myeloid cells, including monocytes and neutrophils (PMID: 12393390). LILRA5 may play a role in triggering innate immune responses. It does not function in class I MHC antigens recognition (PMID: 16675463).

实验方案

Product Specific Protocols
FC protocol for LILRA5/CD85f antibody 98477-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols