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验证数据展示

  • FC experiment of mouse splenocytes using 98420-3-RR

    FC experiment of mouse splenocytes using 98420-3-RR

    1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CRTAM/CD355 Rabbit RecAb (98420-3-RR, Clone: 250055A1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD8a (53-6.7) (APC-65069, Clone: 53-6.7). Cells were not fixed.

  • FC experiment of mouse splenocytes using 98420-3-RR

    FC experiment of mouse splenocytes using 98420-3-RR

    1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CRTAM/CD355 Rabbit RecAb (98420-3-RR, Clone: 250055A1) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse Crtam(CD355 ) Rabbit RecAb (98420-3-RR, Clone:250055A1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were not fixed.

Anti-Mouse CRTAM/CD355 Rabbit Recombinant Antibody

CRTAM/CD355 Uni-rAb® Recombinant Antibody for FC
Cat No. 98420-3-RR

产品说明书

CloneNo. 250055A1

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

CD355, Class-I MHC-restricted T-cell-associated molecule, Crtam, Cytotoxic and regulatory T-cell molecule

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inAnti-CD3/CD28 treated mouse splenocytes

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98420-3-RR targets CRTAM/CD355 in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原

CatNo: Eg2691

Product name: Recombinant Mouse CRTAM/CD355 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 17-284 aa of NM_001281954.1

Sequence: AFLKMETVTVEEGQTLTLTCVTSQTKNVSLQWLAPSGFTIFLNQHPALKSSKYQLLHHSATQLSISVSNVTLREEGVYTCLHYGSSVKTKQVRVTVLVTPFQPTVEALVLRRQNGEKSVVLKCSTERSKPPPQITWLLGEGLEIYGELNHEFEADGKICNTSSMLIARAYGKNSTVHCIIQHEGLHGRKLVAPFQFEDLVADQETSDQETSDAPEQSSLSSQALQQPTSTVSMMENSSIPETDKEEKEHATQDPGLSTEASAQHTGLA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cytotoxic and regulatory T cell molecule
别名 CD355, Class-I MHC-restricted T-cell-associated molecule, Crtam, Cytotoxic and regulatory T-cell molecule
计算分子量44 kDa
GenBank蛋白编号NM_001281954.1
基因名称 Crtam
Gene ID (NCBI) 54698
RRIDAB_3746061
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ149L7-1
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

Cytotoxic and regulatory T cell molecule (CRTAM, also known as CD355) is an immunoglobulin-superfamily transmembrane protein with V and C1-like Ig domains, which is involved in epithelial cell adhesion (PMID: 18329370; 20556794). CRTAM is critical to instruct the differentiation of CD4+ cytotoxic T cells (CTL) through the induction of eomesodermin and CTL-related genes (PMID: 26694968). It also regulates CD8+ T cell retention within the lymph node and eventually effector function (PMID: 19752223).

实验方案

Product Specific Protocols
FC protocol for CRTAM/CD355 antibody 98420-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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