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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • FC experiment of Transfected HEK-293T using 98417-2-RR

    FC experiment of Transfected HEK-293T using 98417-2-RR

    1x10^6 HEK-293T cells (left) or CXCL5-transfected HEK-293T cells (right) with 0.25 ug Anti-Mouse CXCL5 Rabbit RecAb (98417-2-RR, Clone: 242737D4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of Transfected HEK-293T using 98417-2-RR

    FC experiment of Transfected HEK-293T using 98417-2-RR

    1x10^6 HEK-293T cells (right) or CXCL5-transfected HEK-293T cells (left) with 0.25 ug Anti-Mouse CXCL5 Rabbit RecAb (98417-2-RR, Clone: 242737D4) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-2-RR, Clone: 240953A11) (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Anti-Mouse CXCL5 Rabbit Recombinant Antibody

CXCL5 Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. 98417-2-RR

产品说明书

CloneNo. 242737D4

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC (Intra)

别名

C-X-C motif chemokine 5, Cytokine LIX, GCP-2(1-78), GCP-2(9-78), Scyb5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inTransfected HEK-293T cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98417-2-RR targets CXCL5 in FC (Intra) applications and shows reactivity with mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 mouse
免疫原 CXCL5 fusion protein Eg1636 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chemokine (C-X-C motif) ligand 5
别名 C-X-C motif chemokine 5, Cytokine LIX, GCP-2(1-78), GCP-2(9-78), Scyb5
计算分子量14KD
GenBank蛋白编号NM_009141.3
基因名称 Cxcl5
Gene ID (NCBI) 20311
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP50228
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

Chemokines are a class of pro-inflammatory cytokines that can recruit and activate chemotactic cells. CXCL5 is a member of the chemokine family binding CXCR2, a G-protein coupled receptor. CXCL5 is a chemokine that promotes tumor formation by triggering the migration of immune cells to tumors and promotes immmuno-suppressive characteristics of the tumor microenvironment. CXCL5 can also promote tumor cell metastasis and recruit vascular endothelial cells for angiogenesis.

实验方案

Product Specific Protocols
FC protocol for CXCL5 antibody 98417-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols