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验证数据展示

  • FC experiment of Transfected HEK-293T using 98388-1-RR

    FC experiment of Transfected HEK-293T using 98388-1-RR

    1x10^6 HEK-293T cells(left) and Il28a-transfected HEK-293T cells(right) were stained with 0.25 ug Anti-Mouse IL-28A Rabbit RecAb (98388-1-RR, Clone:242247E2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L).Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of Transfected HEK-293T using 98388-1-RR

    FC experiment of Transfected HEK-293T using 98388-1-RR

    1x10^6 HEK-293T cells(left) and Il28a-transfected HEK-293T cells(right) were stained with 0.25 ug Anti-Mouse IL-28A Rabbit RecAb (98388-1-RR, Clone:242247E2)(red), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and APC-Conjugated Goat Anti-Rabbit IgG(H+L).Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Anti-Mouse IL-28A Rabbit Recombinant Antibody

IL-28A Uni-rAbTM Recombinant Antibody for FC
Cat No. 98388-1-RR

产品说明书

CloneNo. 242247E2

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

Ifnl2, IFN-lambda-2, Il28a, Interferon lambda-2, Interleukin-28A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inTransfected HEK-293T cells

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98388-1-RR targets IL-28A in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原 IL-28A fusion protein Eg3169 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 28A
别名 Ifnl2, IFN-lambda-2, Il28a, Interferon lambda-2, Interleukin-28A
计算分子量22 kDa
GenBank蛋白编号NM_001024673.2
基因名称 Il28a
Gene ID (NCBI) 330496
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ4VK74
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

IL28A, also named as IFNL2 and ZCYT020, is a cytokine with immunomodulatory activity. It up-regulates MHC class I antigen expression. IL28A is a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1. The ligand/receptor complex seems to signal through the Jak-STAT pathway. It plays a significant role in the antiviral immune defense in the intestinal epithelium.

实验方案

Product Specific Protocols
FC protocol for IL-28A antibody 98388-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols