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验证数据展示

  • FC experiment of Transfected HEK-293T using 98382-2-RR

    FC experiment of Transfected HEK-293T using 98382-2-RR

    1x10^6 HEK-293T cells or TWEAKR/CD266 transfected HEK-293T cells were stained with 0.25 ug Anti-Mouse TWEAK R/TNFRSF12 Rabbit RecAb (98382-2-RR, Clone:241646F11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • FC experiment of Transfected HEK-293T using 98382-2-RR

    FC experiment of Transfected HEK-293T using 98382-2-RR

    1x10^6 HEK-293T cells(left) or TWEAK R/TNFRSF12 transfected HEK-293T cells(right) were stained with 0.25 ug Anti-Mouse TWEAK R/TNFRSF12 Rabbit RecAb (98382-2-RR, Clone:241646F11)(red) , or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 98382-2-RR

    Affinity and Kinetic Characterization of 98382-2-RR

    Biolayer interferometry (BLl) kinetic assays of 98382-2-RR against Mouse TWEAKR/CD266 were performed. The affinity constant is 0.983 nM.

Anti-Mouse TWEAKR/CD266 Rabbit Recombinant Antibody

TWEAKR/CD266 Uni-rAb® Recombinant Antibody for FC
Cat No. 98382-2-RR

产品说明书

CloneNo. 241646F11

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

TNFRSF12, TWEAK R, CD266, FGF-inducible 14, Fgfrp2

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inTransfected HEK-293T cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98382-2-RR targets TWEAKR/CD266 in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原

CatNo: Eg1838

Product name: Recombinant Mouse TWEAKR/CD266 protein (rFc Tag) (HPLC-verified)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 28-80 aa of NM_013749.2

Sequence: EQAPGTSPCSSGSSWSADLDKCMDCASCPARPHSDFCLGCAAAPPAHFRLLWP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor necrosis factor receptor superfamily, member 12a
别名 TNFRSF12, TWEAK R, CD266, FGF-inducible 14, Fgfrp2
计算分子量14kDa
GenBank蛋白编号NM_013749.2
基因名称 Tnfrsf12a
Gene ID (NCBI) 27279
RRIDAB_3746027
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9CR75
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

TWEAKR (also known as CD266, FN14, TNFRSF12A) is a member of the TNF receptor superfamily which is activated by its ligand, the cytokine TWEAK (TNFSF12). TWEAKR is the smallest member of the TNFR superfamily. TweakR was first described as an FGF-inducible gene that played a role in fibroblast adhesion and migration. Subsequently, the induction of TweakR expression by other growth factors and/or upon tissue injury was observed in multiple cell types, including hepatocytes, endothelial cells, adipocytes, and cardiomyocytes. (PMID: 23073510, PMID: 24409185)

实验方案

Product Specific Protocols
FC protocol for TWEAKR/CD266 antibody 98382-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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