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验证数据展示

  • FC experiment of Transfected HEK-293T using 98331-2-RR

    FC experiment of Transfected HEK-293T using 98331-2-RR

    1x10^6 HEK-293T cells or APRIL/TNFSF13 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse APRIL/TNFSF13 Rabbit RecAb (98331-2-RR, Clone: 241666F9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • FC experiment of Transfected HEK-293T using 98331-2-RR

    FC experiment of Transfected HEK-293T using 98331-2-RR

    1x10^6 HEK-293T cells or APRIL/TNFSF13 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse APRIL/TNFSF13 Rabbit RecAb (98331-2-RR, Clone:241666F9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 98331-2-RR

    Affinity and Kinetic Characterization of 98331-2-RR

    Biolayer interferometry (BLl) kinetic assays of 98331-2-RR against Mouse APRIL/TNFSF13 were performed. The affinity constant is 0.228 nM.

Anti-Mouse APRIL/TNFSF13 Rabbit Recombinant Antibody

APRIL/TNFSF13 Uni-rAb® Recombinant Antibody for FC (Intra)
Cat No. 98331-2-RR

产品说明书

CloneNo. 241666F9

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC (Intra)

别名

241666F9, A proliferation-inducing ligand, APRIL, CD256, Tnfsf13

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inTransfected HEK-293T cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98331-2-RR targets APRIL/TNFSF13 in FC (Intra) applications and shows reactivity with mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 mouse
免疫原

CatNo: Eg1561

Product name: Recombinant Mouse APRIL/TNFSF13 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-N-rFc

Tag: N-rFc

Domain: 96-241 aa of NM_023517.2

Sequence: AVLTQKHKKKHSVLHLVPVNITSKADSDVTEVMWQPVLRRGRGLEAQGDIVRVWDTGIYLLYSQVLFHDVTFTMGQVVSREGQGRRETLFRCIRSMPSDPDRAYNSCYSAGVFHLHQGDIITVKIPRANAKLSLSPHGTFLGFVKL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor necrosis factor (ligand) superfamily, member 13
别名 241666F9, A proliferation-inducing ligand, APRIL, CD256, Tnfsf13
计算分子量27kDa
GenBank蛋白编号NM_023517.2
基因名称 Tnfsf13
Gene ID (NCBI) 69583
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9D777
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

TNFSF13 gene encodes tumor necrosis factor ligand superfamily member 13 (or APRIL) belonging to the tumor necrosis factor (TNF) family. APRIL is highly expressed in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages, and its precursor is cleaved by furin. APRIL may be implicated in the regulation of tumor cell growth and also monocyte/macrophage-mediated immunological processes.

实验方案

Product Specific Protocols
FC protocol for APRIL/TNFSF13 antibody 98331-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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