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验证数据展示

  • FC experiment of human peripheral blood leukocytes using 98271-1-RR

    FC experiment of human peripheral blood leukocytes using 98271-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human IL-17RA/CD217 Rabbit Recombinant Antibody (98271-1-RR, Clone: 241891G7) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD16 (3G8). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using 98271-1-RR

    FC experiment of human peripheral blood leukocytes using 98271-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human IL-17RA/CD217 Rabbit Recombinant Antibody (98271-1-RR, Clone: 241891G7) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98271-1-RR

    Affinity and Kinetic Characterization of 98271-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98271-1-RR against Human IL-17RA/CD217 were performed. The affinity constant is 0.115 nM.

Anti-Human IL-17RA Rabbit Recombinant Antibody

IL-17RA Recombinant Antibody for FC
Cat No. 98271-1-RR

产品说明书

CloneNo. 241891G7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

CD217, IL17RA, 241891G7, CANDF5, CDw217

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98271-1-RR targets IL-17RA in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 IL-17RA fusion protein Eg2317 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 17 receptor A
别名 CD217, IL17RA, 241891G7, CANDF5, CDw217
计算分子量 866 aa, 96 kDa
GenBank蛋白编号BC011624
基因名称 IL17RA
Gene ID (NCBI) 23765
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ96F46
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

Interleukin-17 receptor A (IL17A) is a major effector cytokine of the innate and adaptive immune system and is involved in antimicrobial host defense and maintenance of tissue integrity. It has been shown that IL-17A is involved in the pathogenesis of asthma and allergy and regulates local immune and inflammatory processes in bone tissue and is involved in the onset and progression of osteoporosis. In addition to this, due to polymorphisms in the IL17RA gene, it leads to the development of Fanconi anemia.

实验方案

Product Specific Protocols
FC protocol for IL-17RA antibody 98271-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols