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验证数据展示

  • FC experiment of human PBMCs using 98234-1-RR

    FC experiment of human PBMCs using 98234-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IL-2RG/CD132 Rabbit RecAb (98234-1-RR, Clone: 242020H6) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD4 Rabbit Recombinant Antibody (APC-98042, Clone: 240427E12). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using 98234-1-RR

    FC experiment of human PBMCs using 98234-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IL-2RG/CD132 Rabbit RecAb (98234-1-RR, Clone: 242020H6) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98234-1-RR

    Affinity and Kinetic Characterization of 98234-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98234-1-RR against Human IL-2RG/CD132 were performed. The affinity constant is 1.55 nM.

Anti-Human IL-2RG/CD132 Rabbit Recombinant Antibody

IL-2RG/CD132 Uni-rAb® Recombinant Antibody for FC
Cat No. 98234-1-RR

产品说明书

CloneNo. 242020H6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

IL2RG, 242020H6, CD132, gammaC, IL 2 receptor subunit gamma

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98234-1-RR targets IL-2RG/CD132 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

CatNo: Eg1705

Product name: Recombinant Human IL-2 R gamma/CD132 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 23-254 aa of NM_000206.3

Sequence: LNTTILTPNGNEDTTADFFLTTMPTDSLSVSTLPLPEVQCFVFNVEYMNCTWNSSSEPQPTNLTLHYWYKNSDNDKVQKCSHYLFSEEITSGCQLQKKEIHLYQTFVVQLQDPREPRRQATQMLKLQNLVIPWAPENLTLHKLSESQLELNWNNRFLNHCLEHLVQYRTDWDHSWTEQSVDYRHKFSLPSVDGQKRYTFRVRSRFNPLCGSAQHWSEWSHPIHWGSNTSKEN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 2 receptor, gamma (severe combined immunodeficiency)
别名 IL2RG, 242020H6, CD132, gammaC, IL 2 receptor subunit gamma
计算分子量NM_000206.3
GenBank蛋白编号NM_000206.3
基因名称 IL2RG
Gene ID (NCBI) 3561
RRIDAB_3745901
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP31785-1
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

The interleukin-2 receptor (IL-2R) is a heterotrimeric receptor consisting of three subunits, alpha (IL-2RA), beta (IL-2RB), and gamma (IL-2RG). IL-2RG, also known as CD132, is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in the gene of IL-2RG cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.

实验方案

Product Specific Protocols
FC protocol for IL-2RG/CD132 antibody 98234-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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