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验证数据展示

  • FC experiment of mouse splenocytes using 98213-1-RR

    FC experiment of mouse splenocytes using 98213-1-RR

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD157 Rabbit Recombinant Antibody (98213-1-RR, Clone: 241822H1) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD3 (17A2) (APC-65077, Clone: 17A2). Cells were not fixed.

  • FC experiment of mouse splenocytes using 98213-1-RR

    FC experiment of mouse splenocytes using 98213-1-RR

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD157 Rabbit Recombinant Antibody (98213-1-RR, Clone: 241822H1) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98213-1-RR

    Affinity and Kinetic Characterization of 98213-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98213-1-RR against Mouse CD157 were performed. The affinity constant is 0.904 nM.

Anti-Mouse CD157 Rabbit Recombinant Antibody

CD157 Recombinant Antibody for FC
Cat No. 98213-1-RR

产品说明书

CloneNo. 241822H1

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

Bp3, Bone marrow stromal antigen 1, Antigen BP3, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2, ADP-ribosyl cyclase 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inmouse splenocytes

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98213-1-RR targets CD157 in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 bone marrow stromal cell antigen 1
别名 Bp3, Bone marrow stromal antigen 1, Antigen BP3, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2, ADP-ribosyl cyclase 2
计算分子量35kDa
基因名称 Bst1
Gene ID (NCBI) 12182
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ64277
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD157, also known as bone marrow stromal cell antigen 1 (BST-1), or ADP-ribosyl cyclase 2, is a glycosyl phosphotidylinositol-anchored protein that stimulates pre-B-cell growth and has adenosine diphosphate (ADP)-ribosyl cyclase and cyclic ADP-ribose (cADPR) hydrolase activity (PMID: 9473467, 11866528). The two enzymatic activities are responsible for the synthesis and hydrolysis of cADPR, a novel second messenger of calcium release from intracellular calcium stores.

实验方案

Product Specific Protocols
FC protocol for CD157 antibody 98213-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols