验证数据展示
经过测试的应用
| Positive FC detected in | human peripheral blood leukocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
98177-1-RR targets Siglec-8 in FC applications and shows reactivity with human samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | human |
| 免疫原 |
Recombinant Protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | sialic acid binding Ig-like lectin 8 |
| 别名 | SIGLEC8, SIGLEC 8, Sialoadhesin family member 2, Sialic acid-binding Ig-like lectin 8, SAF2 |
| 计算分子量 | 499 aa, 54 kDa |
| GenBank蛋白编号 | BC053319 |
| 基因名称 | SIGLEC8 |
| Gene ID (NCBI) | 27181 |
| RRID | AB_3672320 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q9NYZ4 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2 - 8°C. Stable for one year after shipment. |
背景介绍
Sialic acid-binding Ig-like lectin 8 (Siglec-8), also known as SAF2, is a sialic acid-binding immunoglobulin-like lectin that belongs to the SIGLEC family of the Ig superfamily (PMID: 10856141). Siglec-8 is a single-pass transmembrane protein containing three extracellular immunoglobulin-like domains, a transmembrane region, and a cytoplasmic tail (PMID: 10625619). It is an inhibitory receptor selectively expressed on human mast cells and eosinophils, and at low levels on basophils (PMID: 10856141; 33374255). Engagement of Siglec-8 with antibody results in inhibiting IgE-mediated mast cell responses and reducing allergic inflammation (PMID: 32814824).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for Siglec-8 antibody 98177-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
