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验证数据展示

  • FC experiment of human PBMCs using 98131-1-RR

    FC experiment of human PBMCs using 98131-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD47 Rabbit RecAb (98131-1-RR, Clone: 240997D8) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using 98131-1-RR

    FC experiment of human PBMCs using 98131-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD47 Rabbit RecAb (98131-1-RR, Clone: 240997D8) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98131-1-RR

    Affinity and Kinetic Characterization of 98131-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98131-1-RR against Human CD47 were performed. The affinity constant is 0.723 nM.

Anti-Human CD47 Rabbit Recombinant Antibody

CD47 Uni-rAb® Recombinant Antibody for FC
Cat No. 98131-1-RR

产品说明书

CloneNo. 240997D8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

240997D8, CD47 molecule, IAP, Integrin associated protein, Integrin-associated protein

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98131-1-RR targets CD47 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

CatNo: Eg31499

Product name: Recombinant Human CD47 protein (Myc Tag, His Tag)

Source: mammalian cells-derived, pHZ-KIsec

Tag: Myc & 6*His

Domain: 19-139 aa of BC010016

Sequence: QLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVCVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD47 molecule
别名 240997D8, CD47 molecule, IAP, Integrin associated protein, Integrin-associated protein
计算分子量 323 aa, 35 kDa
GenBank蛋白编号BC010016
基因名称 CD47
Gene ID (NCBI) 961
RRIDAB_3672277
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ08722
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts as a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.

实验方案

Product Specific Protocols
FC protocol for CD47 antibody 98131-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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