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验证数据展示

  • FC experiment of mouse splenocytes using 98119-1-RR

    FC experiment of mouse splenocytes using 98119-1-RR

    1x10^6 untreated or LPS-treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD83 Rabbit Recombinant Antibody (98119-1-RR, Clone: 241002B7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • FC experiment of mouse splenocytes using 98119-1-RR

    FC experiment of mouse splenocytes using 98119-1-RR

    1x10^6 LPS-treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD83 Rabbit Recombinant Antibody (98119-1-RR, Clone: 241002B7) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD83 Rabbit Recombinant Antibody (98119-1-RR, Clone: 241002B7) (black, dashed), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98119-1-RR

    Affinity and Kinetic Characterization of 98119-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98119-1-RR against Mouse CD83 were performed. The affinity constant is 0.199 nM.

Anti-Mouse CD83 Rabbit Recombinant Antibody

CD83 Uni-rAb® Recombinant Antibody for FC
Cat No. 98119-1-RR

产品说明书

CloneNo. 241002B7

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

241002B7, CD83 antigen, mCD83

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inLPS treated mouse splenocytes

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98119-1-RR targets CD83 in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原

CatNo: Eg1387

Product name: Recombinant Mouse CD83 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 22-133 aa of NM_009856.3

Sequence: MREVTVACSETADLPCTAPWDPQLSYAVSWAKVSESGTESVELPESKQNSSFEAPRRRAYSLTIQNTTICSSGTYRCALQELGGQRNLSGTVVLKVTGCPKEATESTFRKYR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD83 antigen
别名 241002B7, CD83 antigen, mCD83
计算分子量21 kDa
GenBank蛋白编号NM_009856.3
基因名称 Cd83
Gene ID (NCBI) 12522
RRIDAB_3672266
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO88324
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs) (PMID: 17334966). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils (PMID: 31231400; 17334966). CD83 regulates the maturation, activation, and homeostasis of numerous immune cells (PMID: 31231400; 32362900). CD83 can also expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation (PMID: 17334966; 12403928).

实验方案

Product Specific Protocols
FC protocol for CD83 antibody 98119-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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