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验证数据展示

  • FC experiment of human PBMCs using 98115-1-RR

    FC experiment of human PBMCs using 98115-1-RR

    1x10^6 untreated or anti-CD3/CD28 treated human PBMCs were surface stained with 0.25 ug Anti-Human TIM3 Rabbit RecAb (98115-1-RR, Clone: 241422H1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD4 (OKT4) (APC-65134, Clone: OKT4). Cells were not fixed.

  • FC experiment of human PBMCs using 98115-1-RR

    FC experiment of human PBMCs using 98115-1-RR

    1x10^6 anti-CD3/CD28 treated human PBMCs were surface stained with 0.25 ug Anti-Human TIM3 Rabbit Recombinant Antibody (98115-1-RR, Clone: 241422H1) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human TIM3 Rabbit Recombinant Antibody (98115-1-RR, Clone: 241422H1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98115-1-RR

    Affinity and Kinetic Characterization of 98115-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98115-1-RR against Human TIM3 were performed. The affinity constant is 0.688 nM.

Anti-Human TIM3 Rabbit Recombinant Antibody

TIM3 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98115-1-RR

产品说明书

CloneNo. 241422H1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

241422H1, CD366, HAVCR2, HAVcr-2, T-cell immunoglobulin and mucin domain-containing protein 3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inAnti-CD3/CD28 treated human PBMCs

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98115-1-RR targets TIM3 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hepatitis A virus cellular receptor 2
别名 241422H1, CD366, HAVCR2, HAVcr-2, T-cell immunoglobulin and mucin domain-containing protein 3
计算分子量 301 aa, 33 kDa
GenBank蛋白编号BC020843
基因名称 TIM3
Gene ID (NCBI) 84868
RRIDAB_3672262
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ8TDQ0
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920).

实验方案

Product Specific Protocols
FC protocol for TIM3 antibody 98115-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols