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验证数据展示

  • FC experiment of mouse splenocytes using 98024-1-RR (same clone as 98024-1-PBS)

    FC experiment of mouse splenocytes using 98024-1-RR (same clone as 98024-1-PBS)

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD126/IL-6R alpha Rabbit Recombinant Antibody (98024-1-RR, Clone: 230371A7) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were not fixed. This data was developed using the same antibody clone with 98024-1-PBS in a different storage buffer formulation.

  • FC experiment of mouse splenocytes using 98024-1-RR (same clone as 98024-1-PBS)

    FC experiment of mouse splenocytes using 98024-1-RR (same clone as 98024-1-PBS)

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD126/IL-6R alpha Rabbit Recombinant Antibody (98024-1-RR, Clone:230371A7) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98024-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00182-2

    Sandwich ELISA standard curve of MP00182-2

    Sandwich ELISA standard curve of MP00182-2, Mouse CD126/IL-6R alpha Monoclonal Matched Antibody Pair - PBS only. 98024-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0664. 83209-2-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL.

  • Sample test of MP00182-2

    Sample test of MP00182-2

    J774A.1 mouse reticulum cell sarcoma macrophage cells (1 x 106 cells/mL) were cultured for 3 days in DMEM containing 10% fetal bovine serum. An aliquot of the cell culture supernatants were removed, assayed for mouse IL-6R alpha, and measured 402.5 pg/mL. Liver tissue from one mouse was homogenized and seeded into 100 mL of RPMI containing 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin sulfate, and 50 μM β-mercaptoethanol for 3 days. An aliquot of the cell culture supernate was removed, assayed for mouse IL-6R alpha, and measured 345.1 pg/mL.

  • Sample test of MP00182-2

    Sample test of MP00182-2

    Serum of sixteen mice were measured. The IL-6R alpha concentration of detected samples was determined to be 10,180.3 pg/mL with a range of 6,065.2-15,362.2 pg/mL.

  • Affinity and Kinetic Characterization of 98024-1-RR

    Affinity and Kinetic Characterization of 98024-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98024-1 -RR against Mouse IL-6R alpha were performed. The affinity constant is 0.629 nM.

Anti-Mouse CD126/IL-6R alpha Rabbit Recombinant Antibody, PBS Only (Capture)

CD126/IL-6R alpha Uni-rAb® Recombinant Antibody for FC, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 98024-1-PBS

产品说明书

CloneNo. 230371A7

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC, Sandwich ELISA, Indirect ELISA, Sample test

别名

Il6ra, CD126, 230371A7, IL-6R alpha, Il6r

缓冲液配方:  PBS Only
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

98024-1-PBS targets CD126/IL-6R alpha in FC, Sandwich ELISA, Indirect ELISA, Sample test applications and shows reactivity with mouse samples.

经测试应用 FC, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 mouse
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 6 receptor, alpha
别名 Il6ra, CD126, 230371A7, IL-6R alpha, Il6r
计算分子量50KD
GenBank蛋白编号NM_010559.3
基因名称 Il6ra
Gene ID (NCBI) 16194
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP22272
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R alpha, the complex of IL-6 and IL-6R alpha associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while expression of IL-6R alpha is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892).

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