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验证数据展示

  • WB analysis using 88615-1-RR (same clone as 88615-1-PBS)

    WB analysis using 88615-1-RR (same clone as 88615-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88615-1-RR (Calbindin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88615-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse cerebellum using 88615-1-RR (same clone as 88615-1-PBS)

    WB analysis of mouse cerebellum using 88615-1-RR (same clone as 88615-1-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 88615-1-RR (Calbindin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88615-1-PBS in a different storage buffer formulation.

  • IHC staining of rat cerebellum using 88615-1-RR (same clone as 88615-1-PBS)

    IHC staining of rat cerebellum using 88615-1-RR (same clone as 88615-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 88615-1-RR (Calbindin-D28k antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88615-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Calbindin-D28k antibody included
  • IHC staining of rat cerebellum using 88615-1-RR (same clone as 88615-1-PBS)

    IHC staining of rat cerebellum using 88615-1-RR (same clone as 88615-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 88615-1-RR (Calbindin-D28k antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88615-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Calbindin-D28k antibody included
  • IF Staining of mouse cerebellum using 88615-1-RR (same clone as 88615-1-PBS)

    IF Staining of mouse cerebellum using 88615-1-RR (same clone as 88615-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using Calbindin-D28k antibody (88615-1-RR, Clone: 261340A1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88615-1-PBS in a different storage buffer formulation.

  • WB analysis of pig brain using 88615-1-RR (same clone as 88615-1-PBS)

    WB analysis of pig brain using 88615-1-RR (same clone as 88615-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 88615-1-RR (Calbindin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88615-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 88615-1-RR (same clone as 88615-1-PBS)

    IHC staining of mouse cerebellum using 88615-1-RR (same clone as 88615-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 88615-1-RR (Calbindin-D28k antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88615-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Calbindin-D28k antibody included
  • Cytometric bead array sample test of MP03605-1

    Cytometric bead array sample test of MP03605-1

    The mean Calbindin-D28k concentration was determined to be 36.7 ng/mL in human urine .

  • Cytometric bead array standard curve of MP03605-1

    Cytometric bead array standard curve of MP03605-1

    Cytometric bead array standard curve of MP03605-1, Calbindin-D28k Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 88615-2-PBS. Detection antibody: 88615-1-PBS. Standard: Ag5861. Range: 0.469-60 ng/mL.

  • Affinity of 88615-1-RR

    Affinity of 88615-1-RR

    Biolayer interferometry (BLI) kinetic assays of 88615-1-RR against Human Calbindin-D28k were performed. The affinity constant is 2.13 nM.

  • IHC staining of mouse cerebellum using 88615-1-RR (same clone as 88615-1-PBS)

    IHC staining of mouse cerebellum using 88615-1-RR (same clone as 88615-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 88615-1-RR (Calbindin-D28k antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88615-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Calbindin-D28k antibody included

Calbindin-D28k Recombinant monoclonal antibody, PBS Only (Detector)

Calbindin-D28k Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 88615-1-PBS

产品说明书

CloneNo. 261340A1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

别名

CALB1, Calbindin, CALB, calbindin 1, 28kDa, Calbindin D28

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy Calbindin-D28k Ready-To-Use IHC Kit. Calbindin-D28k primary antibody included.

产品信息

88615-1-PBS targets Calbindin-D28k as part of a matched antibody pair:

MP03605-1: 88615-2-PBS capture and 88615-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag5861

Product name: Recombinant human Calbindin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 110-261 aa of BC006478

Sequence: YDTDHSGFIETEELKNFLKDLLEKANKTVDDTKLAEYTDLMLKLFDSNNDGKLELTEMARLLPVQENFLLKFQGIKMCGKEFNKAFELYDQDGNGYIDENELDALLKDLCEKNKQDLDINNITTYKKNIMALSDGGKLYRTDLALILCAGDN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 calbindin 1, 28kDa
别名 CALB1, Calbindin, CALB, calbindin 1, 28kDa, Calbindin D28
计算分子量 30 kDa
观测分子量28 kDa
GenBank蛋白编号BC006478
基因名称 Calbindin
Gene ID (NCBI) 793
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP05937
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.

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