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验证数据展示

  • WB analysis using 88615-1-RR

    WB analysis using 88615-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88615-1-RR (Calbindin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse cerebellum using 88615-1-RR

    WB analysis of mouse cerebellum using 88615-1-RR

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 88615-1-RR (Calbindin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat cerebellum using 88615-1-RR

    IHC staining of rat cerebellum using 88615-1-RR

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 88615-1-RR (Calbindin-D28k antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Calbindin-D28k antibody included
  • IHC staining of rat cerebellum using 88615-1-RR

    IHC staining of rat cerebellum using 88615-1-RR

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 88615-1-RR (Calbindin-D28k antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Calbindin-D28k antibody included
  • IF Staining of mouse cerebellum using 88615-1-RR

    IF Staining of mouse cerebellum using 88615-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using Calbindin-D28k antibody (88615-1-RR, Clone: 261340A1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of pig brain using 88615-1-RR

    WB analysis of pig brain using 88615-1-RR

    pig brain tissue were subjected to SDS PAGE followed by western blot with 88615-1-RR (Calbindin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 88615-1-RR

    IHC staining of mouse cerebellum using 88615-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 88615-1-RR (Calbindin-D28k antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Calbindin-D28k antibody included
  • Affinity of 88615-1-RR

    Affinity of 88615-1-RR

    Biolayer interferometry (BLI) kinetic assays of 88615-1-RR against Human Calbindin-D28k were performed. The affinity constant is 2.13 nM.

  • IHC staining of mouse cerebellum using 88615-1-RR

    IHC staining of mouse cerebellum using 88615-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 88615-1-RR (Calbindin-D28k antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Calbindin-D28k antibody included

Calbindin-D28k Recombinant monoclonal antibody

Calbindin-D28k Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 88615-1-RR

产品说明书

CloneNo. 261340A1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF-P, ELISA

别名

CALB1, Calbindin, CALB, calbindin 1, 28kDa, Calbindin D28

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, pig brain tissue, rat brain tissue, fetal human brain tissue
Positive IHC detected inrat cerebellum tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue
Planning an IHC experiment? We recommend our IHCeasy Calbindin-D28k Ready-To-Use IHC Kit. Calbindin-D28k primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

88615-1-RR targets Calbindin-D28k in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag5861

Product name: Recombinant human Calbindin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 110-261 aa of BC006478

Sequence: YDTDHSGFIETEELKNFLKDLLEKANKTVDDTKLAEYTDLMLKLFDSNNDGKLELTEMARLLPVQENFLLKFQGIKMCGKEFNKAFELYDQDGNGYIDENELDALLKDLCEKNKQDLDINNITTYKKNIMALSDGGKLYRTDLALILCAGDN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 calbindin 1, 28kDa
别名 CALB1, Calbindin, CALB, calbindin 1, 28kDa, Calbindin D28
计算分子量 30 kDa
观测分子量28 kDa
GenBank蛋白编号BC006478
基因名称 Calbindin
Gene ID (NCBI) 793
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP05937
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.

实验方案

Product Specific Protocols
IF protocol for Calbindin-D28k antibody 88615-1-RRDownload protocol
IHC protocol for Calbindin-D28k antibody 88615-1-RRDownload protocol
WB protocol for Calbindin-D28k antibody 88615-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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