Various lysates were subjected to SDS PAGE followed by western blot with 88331-1-RR (PRPF19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88331-1-PBS in a different storage buffer formulation.

The mean PRPF19 concentration was determined to be 545.95 ng/mL in HL-60 cell extract based on a 1.20 mg/mL extract load, 677.04 ng/mL in Raji cell extract based on a 1.50 mg/mL extract load, 371.27 ng/mL in Ramos cell extract based on a 1.20 mg/mL extract load, 278.50 ng/mL in HT-29 cell extract based on a 1.20 mg/mL extract load and 205.25 ng/mL in SKOV-3 cell extract based on a 1.20 mg/mL extract load.

Sandwich ELISA standard curve of MP03457-2, Human PRPF19 Recombinant Matched Antibody Pair - PBS only. 88331-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7700. 88331-4-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 ng/mL.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRPF19 antibody (88331-1-RR, Clone: 260837A11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 88331-1-PBS in a different storage buffer formulation.