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验证数据展示

  • WB analysis of IMR-32 using 88168-3-RR (same clone as 88168-3-PBS)

    WB analysis of IMR-32 using 88168-3-RR (same clone as 88168-3-PBS)

    IMR-32 cells were subjected to SDS PAGE followed by western blot with 88168-3-RR (ACD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88168-3-PBS in a different storage buffer formulation.

  • WB analysis using 88168-3-RR (same clone as 88168-3-PBS)

    WB analysis using 88168-3-RR (same clone as 88168-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88168-3-RR (ACD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88168-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP03378-1

    Sandwich ELISA sample test of MP03378-1

    The mean ACD concentration was determined to be 31.68 ng/mL in Jurkat cell extract based on a 1.20 mg/mL extract load, 11.53 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load, 6.55 ng/mL in Raji cell extract based on a 1.50 mg/mL extract load and 3.19 ng/mL in 786-O cell extract based on a 1.50 mg/mL extract load.

  • Sandwich ELISA standard curve of MP03378-1

    Sandwich ELISA standard curve of MP03378-1

    Sandwich ELISA standard curve of MP03378-1, Human ACD Recombinant Matched Antibody Pair - PBS only. 88168-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag23021. 88168-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

  • Cytometric bead array sample test of MP03378-1

    Cytometric bead array sample test of MP03378-1

    The mean ACD concentration was determined to be 34.1 ng/mL in Jurkat cell extract based on a 1.0 mg/mL extract load, 12.8 ng/mL in HeLa cell extract based on a 1.0 mg/mL extract load, 4.5 ng/mL in Raji cell extract based on a 1.0 mg/mL extract load, 1.2 ng/mL in 786-O cell extract based on a 1.0 mg/mL extract load.

  • Cytometric bead array standard curve of MP03378-1

    Cytometric bead array standard curve of MP03378-1

    Cytometric bead array standard curve of MP03378-1, ACD Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 88168-3-PBS. Detection antibody: 88168-2-PBS. Standard: Ag23021. Range: 0.312-40 ng/mL.

ACD Recombinant monoclonal antibody, PBS Only (Capture)

ACD Uni-rAb® Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 88168-3-PBS

产品说明书

CloneNo. 260314F11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

别名

PIP1, POT1 and TIN2-interacting protein, PTOP, TINT1, TPP1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

88168-3-PBS targets ACD as part of a matched antibody pair:

MP03378-1: 88168-3-PBS capture and 88168-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag23021

Product name: Recombinant human ACD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC016904

Sequence: MPGRCQSDAAMRVNGPASRAPAGWTSGSLHTGPRAGRPRAQARGVRGRGLLLRPRPAKELPLPRKGGAWAPAGNPGPLHPLGVAVGMAGSGRLVLRPWIRELILGSETPSSPRAGQLLEVLQDAEAAVAGPSHAPDTSDVGATLLVSDGTHSVRCLVTREALDTSDWEEKEFGFRGTEGRLLLLQDCGVHVQVAEGGAPAEFYLQVDRFSLLPTEQPRLRVPGCNQDLDVQKKLYDCLEEHLSESTSSNAGLSLSQLLDEMREDQEHQGALVCLAESCLTLEGPCTAPPVTHWAASRCKATGEAVYTVPSSMLCISENDQLILSSLGPCQRTQGPELPPPDPALQDLSLTL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 adrenocortical dysplasia homolog (mouse)
别名 PIP1, POT1 and TIN2-interacting protein, PTOP, TINT1, TPP1
计算分子量 58 kDa
观测分子量60 kDa
GenBank蛋白编号BC016904
基因名称 ACD
Gene ID (NCBI) 65057
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96AP0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACD (Adrenocortical Dysplasia Homolog), also known as TPP1 or POT1- and TIN2-Organizing Protein, is a core component of the telomeric Shelterin complex. It participates in telomere length maintenance, telomere structural protection, and regulation of genomic stability. Together with POT1, TIN2, TRF1, TRF2, RAP1, and TIN2, ACD assembles into the Shelterin complex, which coats telomeric DNA, suppresses DNA damage response and telomere fusion, and prevents chromosome ends from being recognized as DNA double-strand breaks. Mutations in the ACD gene cause Dyskeratosis Congenita, Hoyeraal-Hreidarsson syndrome, and bone marrow failure syndromes, and are closely associated with telomerase dysfunction.

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