验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, IMR-32 cells, SiHa cells, Raji cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
88168-3-RR targets ACD in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag23021 Product name: Recombinant human ACD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC016904 Sequence: MPGRCQSDAAMRVNGPASRAPAGWTSGSLHTGPRAGRPRAQARGVRGRGLLLRPRPAKELPLPRKGGAWAPAGNPGPLHPLGVAVGMAGSGRLVLRPWIRELILGSETPSSPRAGQLLEVLQDAEAAVAGPSHAPDTSDVGATLLVSDGTHSVRCLVTREALDTSDWEEKEFGFRGTEGRLLLLQDCGVHVQVAEGGAPAEFYLQVDRFSLLPTEQPRLRVPGCNQDLDVQKKLYDCLEEHLSESTSSNAGLSLSQLLDEMREDQEHQGALVCLAESCLTLEGPCTAPPVTHWAASRCKATGEAVYTVPSSMLCISENDQLILSSLGPCQRTQGPELPPPDPALQDLSLTL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | adrenocortical dysplasia homolog (mouse) |
| 别名 | PIP1, POT1 and TIN2-interacting protein, PTOP, TINT1, TPP1 |
| 计算分子量 | 58 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC016904 |
| 基因名称 | ACD |
| Gene ID (NCBI) | 65057 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q96AP0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ACD (Adrenocortical Dysplasia Homolog), also known as TPP1 or POT1- and TIN2-Organizing Protein, is a core component of the telomeric Shelterin complex. It participates in telomere length maintenance, telomere structural protection, and regulation of genomic stability. Together with POT1, TIN2, TRF1, TRF2, RAP1, and TIN2, ACD assembles into the Shelterin complex, which coats telomeric DNA, suppresses DNA damage response and telomere fusion, and prevents chromosome ends from being recognized as DNA double-strand breaks. Mutations in the ACD gene cause Dyskeratosis Congenita, Hoyeraal-Hreidarsson syndrome, and bone marrow failure syndromes, and are closely associated with telomerase dysfunction.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ACD antibody 88168-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
