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验证数据展示

  • WB analysis using 88127-2-RR (same clone as 88127-2-PBS)

    WB analysis using 88127-2-RR (same clone as 88127-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88127-2-RR (MELTF/CD228 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88127-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP03370-1

    Sandwich ELISA sample test of MP03370-1

    The mean MFI2 concentration was determined to be 102.73 ng/mL in HEK-293 cell extract based on a 1.50 mg/mL extract load, 8.59 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load and 1.26 ng/mL in HL-60 cell extract based on a 1.20 mg/mL extract load.

  • Sandwich ELISA standard curve of MP03370-1

    Sandwich ELISA standard curve of MP03370-1

    Sandwich ELISA standard curve of MP03370-1, Human MFI2 Recombinant Matched Antibody Pair - PBS only. 88127-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg9197. 88127-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Cytometric bead array sample test of MP03370-1

    Cytometric bead array sample test of MP03370-1

    The mean MFI2 concentration was determined to be 42.3 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load, 3.2 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load, 0.5 ng/mL in Raji cell extract based on a 1.5 mg/mL extract load.

  • Cytometric bead array standard curve of MP03370-1

    Cytometric bead array standard curve of MP03370-1

    Cytometric bead array standard curve of MP03370-1, MFI2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 88127-2-PBS. Detection antibody: 88127-3-PBS. Standard: Eg9197. Range: 0.312-40 ng/mL.

MFI2 Recombinant monoclonal antibody, PBS Only (Capture)

MFI2 Uni-rAb® Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 88127-2-PBS

产品说明书

CloneNo. 260392B10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

别名

Melanoma-associated antigen p97, Melanotransferrin, MELTF

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

88127-2-PBS targets MFI2 as part of a matched antibody pair:

MP03370-1: 88127-2-PBS capture and 88127-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg9197

Product name: Recombinant human MELTF/CD228 protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 20-709 aa of NM_005929.6

Sequence: GMEVRWCATSDPEQHKCGNMSEAFREAGIQPSLLCVRGTSADHCVQLIAAQEADAITLDGGAIYEAGKEHGLKPVVGEVYDQEVGTSYYAVAVVRRSSHVTIDTLKGVKSCHTGINRTVGWNVPVGYLVESGRLSVMGCDVLKAVSDYFGGSCVPGAGETSYSESLCRLCRGDSSGEGVCDKSPLERYYDYSGAFRCLAEGAGDVAFVKHSTVLENTDGKTLPSWGQALLSQDFELLCRDGSRADVTEWRQCHLARVPAHAVVVRADTDGGLIFRLLNEGQRLFSHEGSSFQMFSSEAYGQKDLLFKDSTSELVPIATQTYEAWLGHEYLHAMKGLLCDPNRLPPYLRWCVLSTPEIQKCGDMAVAFRRQRLKPEIQCVSAKSPQHCMERIQAEQVDAVTLSGEDIYTAGKTYGLVPAAGEHYAPEDSSNSYYVVAVVRRDSSHAFTLDELRGKRSCHAGFGSPAGWDVPVGALIQRGFIRPKDCDVLTAVSEFFNASCVPVNNPKNYPSSLCALCVGDEQGRNKCVGNSQERYYGYRGAFRCLVENAGDVAFVRHTTVFDNTNGHNSEPWAAELRSEDYELLCPNGARAEVSQFAACNLAQIPPHAVMVRPDTNIFTVYGLLDKAQDLFGDDHNKNGFKMFDSSNYHGQDLLFKDATVRAVPVGEKTTYRGWLGLDYVAALEGMSSQQC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5
别名 Melanoma-associated antigen p97, Melanotransferrin, MELTF
计算分子量80 kDa
观测分子量 82 kDa
GenBank蛋白编号NM_005929.6
基因名称 MFI2
Gene ID (NCBI) 4241
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP08582-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MFI2 (Melanotransferrin/Mtf/TRMF), also known as p97 or CD228, is an iron-binding glycoprotein belonging to the transferrin family. Alternative RNA splicing yields two forms of different length transcripts, one (the longer) bound to the membrane (mMFI2) and the other (the shorter) secreted to the peripheral environment (sMFI2) such as blood and cell supernatant. MFI2 is upregulated in tumor tissues and has been identified as a serological marker of colorectal cancer (PMID: 25216327).

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