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验证数据展示

  • WB analysis using 87789-1-RR (same clone as 87789-1-PBS)

    WB analysis using 87789-1-RR (same clone as 87789-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87789-1-RR (EDEM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87789-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP03204-1

    Cytometric bead array sample test of MP03204-1

    The mean EDEM1 concentration was determined to be 18.0 ng/mL in L02 cell extract based on a 1.2 mg/mL extract load, Caco-2 cell extract were served as a negative control.

  • Cytometric bead array standard curve of MP03204-1

    Cytometric bead array standard curve of MP03204-1

    Cytometric bead array standard curve of MP03204-1, EDEM1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87789-1-PBS. Detection antibody: 87789-2-PBS. Standard: Ag41790. Range: 0.469-60 ng/mL.

  • Affinity of 87789-1-RR

    Affinity of 87789-1-RR

    Biolayer interferometry (BLI) kinetic assays of 87789-1-RR against Human EDEM1 were performed. The affinity constant is 6.42 nM.

EDEM1 Recombinant monoclonal antibody, PBS Only (Capture)

EDEM1 Uni-rAb® Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 87789-1-PBS

产品说明书

CloneNo. 253376D2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Cytometric bead array, Indirect ELISA

别名

EDEM, ER degradation-enhancing alpha-mannosidase-like protein 1, KIAA0212

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy EDEM1 Ready-To-Use IHC Kit. EDEM1 primary antibody included.

产品信息

87789-1-PBS targets EDEM1 as part of a matched antibody pair:

MP03204-1: 87789-1-PBS capture and 87789-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag41790

Product name: Recombinant human EDEM1 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 564-657 aa of BC019088

Sequence: EDNPVHKSGTRYMFTTEGHIVSVDEHLRELPWKEFFSEEGGQDQGGKSVHRPKPHELKVINSSSNCNRVPDERRYSLPLKSIYMRQIDQMVGLI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ER degradation enhancer, mannosidase alpha-like 1
别名 EDEM, ER degradation-enhancing alpha-mannosidase-like protein 1, KIAA0212
计算分子量 74 kDa
观测分子量 75 kDa
GenBank蛋白编号BC019088
基因名称 EDEM1
Gene ID (NCBI) 9695
RRIDAB_3745750
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ92611
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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