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验证数据展示

  • WB analysis using 87660-2-RR (same clone as 87660-2-PBS)

    WB analysis using 87660-2-RR (same clone as 87660-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87660-2-RR (MEF2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87660-2-PBS in a different storage buffer formulation.

  • WB analysis of THP-1 using 87660-2-RR (same clone as 87660-2-PBS)

    WB analysis of THP-1 using 87660-2-RR (same clone as 87660-2-PBS)

    WB result of MEF2A antibody (87660-2-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-MEF2A transfected THP-1 cells. This data was developed using the same antibody clone with 87660-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP03216-1

    Sandwich ELISA sample test of MP03216-1

    The mean MEF2A concentration was determined to be 4.99 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load.

  • Sandwich ELISA sample test of MP03216-1

    Sandwich ELISA sample test of MP03216-1

    Plasma of six individual healthy human donors was measured. The MEF2A concentration of detected samples was determined to be 0.30 ng/mL with a range of 0.07-0.78 ng/mL.

  • Sandwich ELISA standard curve of MP03216-1

    Sandwich ELISA standard curve of MP03216-1

    Sandwich ELISA standard curve of MP03216-1, Human MEF2A Recombinant Matched Antibody Pair - PBS only. 87660-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag41519. 87660-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • IF Staining of HeLa using 87660-2-RR (same clone as 87660-2-PBS)

    IF Staining of HeLa using 87660-2-RR (same clone as 87660-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MEF2A antibody (87660-2-RR, Clone: 253110B3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87660-2-PBS in a different storage buffer formulation.

MEF2A Recombinant monoclonal antibody, PBS Only (Detector)

MEF2A Uni-rAb® Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA
Cat No. 87660-2-PBS

产品说明书

CloneNo. 253110B3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Sandwich ELISA, Indirect ELISA

别名

ADCAD1, MEF2, myocyte enhancer factor 2A, Myocyte-specific enhancer factor 2A, RSRFC4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87660-2-PBS targets MEF2A as part of a matched antibody pair:

MP03216-1: 87660-3-PBS capture and 87660-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag41519

Product name: Recombinant human MEF2A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 150-499 aa of BC013437

Sequence: SPNALSYTNPGSSLVSPSLAASSTLTDSSMLSPPQTTLHRNVSPGAPQRPPSTGNAGGMLSTTDLTVPNGAGSSPVGNGFVNSRASPNLIGATGANSLGKVMPTKSPPPPGGGNLGMNSRKPDLRVVIPPSSKGMMPPLNTQRISSSQATQPLATPVVSVTTPSLPPQGLVYSAMPTAYNTDYSLTSADLSALQGFNSPGMLSLGQVSAWQQHHLGQAALSSLVAGGQLSQGSNLSINTNQNISIKSEPISPPRDRMTPSGFQQQQQQQQQQQPPPPPQPQPQPPQPQPRQEMGRSPVDSLSSSSSSYDGSDREDPRGDFHSPIVLGRPPNTEDRESPSVKRMRMDAWVT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 myocyte enhancer factor 2A
别名 ADCAD1, MEF2, myocyte enhancer factor 2A, Myocyte-specific enhancer factor 2A, RSRFC4
计算分子量 499 aa, 54 kDa
观测分子量50-70kDa
GenBank蛋白编号BC013437
基因名称 MEF2A
Gene ID (NCBI) 4205
RRIDAB_3745680
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ02078
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MEF2A belongs to a family of DNA binding regulatory proteins. Each of these proteins which belong to the myocyte-specific enhancer factor-2 (MEF2) family binds to the MEF2 target DNA sequence present in the regulatory regions of many, if not all, muscle-specific genes. MEF2A plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. MEF2A also has key roles in cardiac and skeletal muscle development. MEF2A is especially abundant in granule neurons of the cerebellar cortex throughout the period of synaptogenesis. This antibody can detect two bands around 60 kDa to 70 kDa, the larger band may correspond to its post-translational modifications.(PMID: 15483225,16484498, 23486945 )

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