验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, T-47D cells, SK-BR-3 cells, MDCK cells, COS-7 cells, mouse cerebellum tissue, rat cerebellum tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87516-1-RR targets TUBG2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat, canine |
| 免疫原 |
CatNo: Ag27728 Product name: Recombinant human TUBG2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 358-451 aa of BC051890 Sequence: VALSRKSPYLPSAHRVSGLMMANHTSISSLFESSCQQFDKLRKRDAFLEQFRKEDMFKDNFDEMDRSREVVQELIDEYHAATQPDYISWGTQEQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | tubulin, gamma 2 |
| 别名 | Gamma 2 tubulin, Gamma-2-tubulin, GTU-88, Tubulin gamma 2 chain, Tubulin gamma-2 chain |
| 计算分子量 | 451 aa, 51 kDa |
| 观测分子量 | 51 kDa |
| GenBank蛋白编号 | BC051890 |
| 基因名称 | TUBG2 |
| Gene ID (NCBI) | 27175 |
| RRID | AB_3745593 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9NRH3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TUBG2 (γ-tubulin 2) is a member of the tubulin family primarily expressed at the centrioles and spindle poles, and is highly homologous to TUBG1. As a core component of the γ-TuRC (γ-tubulin ring complex), it recruits tubulin dimers by binding to adaptor proteins such as γ-TuNA, providing a template for microtubule nucleation and playing a crucial role in the organization of centrosome microtubules and the proper assembly of the spindle.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for TUBG2 antibody 87516-1-RR | Download protocol |
| IHC protocol for TUBG2 antibody 87516-1-RR | Download protocol |
| WB protocol for TUBG2 antibody 87516-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
