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验证数据展示

  • WB analysis using 87481-2-RR (same clone as 87481-2-PBS)

    WB analysis using 87481-2-RR (same clone as 87481-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87481-2-RR (ITB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87481-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 87481-2-RR (same clone as 87481-2-PBS)

    IHC staining of mouse spleen using 87481-2-RR (same clone as 87481-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 87481-2-RR (CD18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87481-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 87481-2-RR (same clone as 87481-2-PBS)

    IHC staining of mouse spleen using 87481-2-RR (same clone as 87481-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 87481-2-RR (CD18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87481-2-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP03055-1

    Cytometric bead array sample test of MP03055-1

    The mean CD18 concentration was determined to be 6.0 ng/mL in RAW 264.7 cell extract based on a 1.2 mg/mL extract load, 2.9 ng/mL in J774A.1 cell extract based on a 1.3 mg/mL extract load, Neuro-2a cell extract were served as a negative control.

  • Cytometric bead array standard curve of MP03055-1

    Cytometric bead array standard curve of MP03055-1

    Cytometric bead array standard curve of MP03055-1, MOUSE CD18 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87481-3-PBS. Detection antibody: 87481-2-PBS. Standard: Eg10459. Range: 0.156-20 ng/mL.

  • Affinity of 87481-2-RR

    Affinity of 87481-2-RR

    Biolayer interferometry (BLl) kinetic assays of 87481-2-RR against Mouse CD18 were performed. The affinity constant is 14.2 nM.

Mouse CD18 Recombinant monoclonal antibody, PBS Only (Detector)

CD18 Uni-rAb® Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 87481-2-PBS

产品说明书

CloneNo. 252753A4

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

Itgb2, integrin beta 2, LAD, LCAMB, Lfa1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87481-2-PBS targets CD18 as part of a matched antibody pair:

MP03055-1: 87481-3-PBS capture and 87481-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg10459

Product name: Recombinant mouse ITB2 protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-Twin-Strep-Tag

Domain: 10011456 of

Sequence: \

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 integrin beta 2
别名 Itgb2, integrin beta 2, LAD, LCAMB, Lfa1
观测分子量 85-90 kDa
基因名称 CD18
Gene ID (NCBI) 16414
RRIDAB_3745573
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during the immune response, and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).

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