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验证数据展示

  • WB analysis using 87481-1-RR (same clone as 87481-1-PBS)

    WB analysis using 87481-1-RR (same clone as 87481-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87481-1-RR (ITB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87481-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP03055-2

    Sandwich ELISA sample test of MP03055-2

    The mean CD18 concentration was determined to be 3,214.8 pg/mL in RAW 264.7 cell extract based on a 1.2 mg/mL extract load and 5,220.1 pg/mL in J774A.1 cell extract based on a 1.2 mg/mL extract load.

  • Sandwich ELISA standard curve of MP03055-2

    Sandwich ELISA standard curve of MP03055-2

    Sandwich ELISA standard curve of MP03055-2, Mouse CD18 Recombinant Matched Antibody Pair - PBS only. 87481-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg10459. 87481-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-1250 pg/mL.

  • Affinity of 87481-1-RR

    Affinity of 87481-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87481-1-RR against Mouse CD18 were performed. The affinity constant is 5.27 nM.

Mouse CD18 Recombinant monoclonal antibody, PBS Only (Detector)

CD18 Uni-rAb® Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA
Cat No. 87481-1-PBS

产品说明书

CloneNo. 252753H7

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, Sandwich ELISA, Indirect ELISA

别名

Itgb2, integrin beta 2, LAD, LCAMB, Lfa1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87481-1-PBS targets CD18 as part of a matched antibody pair:

MP03055-2: 87481-3-PBS capture and 87481-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg10459

Product name: Recombinant mouse ITB2 protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-Twin-Strep-Tag

Domain: 10011456 of

Sequence: \

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 integrin beta 2
别名 Itgb2, integrin beta 2, LAD, LCAMB, Lfa1
观测分子量 85-90 kDa
基因名称 CD18
Gene ID (NCBI) 16414
RRIDAB_3745572
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP11835
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).

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