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验证数据展示

  • WB analysis using 87377-3-RR (same clone as 87377-3-PBS)

    WB analysis using 87377-3-RR (same clone as 87377-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87377-3-RR (TRIM16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87377-3-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 87377-3-RR (same clone as 87377-3-PBS)

    IF Staining of HeLa using 87377-3-RR (same clone as 87377-3-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TRIM16 antibody (87377-3-RR, Clone: 252519D5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87377-3-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 87377-3-RR (same clone as 87377-3-PBS)

    IF Staining of A549 using 87377-3-RR (same clone as 87377-3-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using TRIM16 antibody (87377-3-RR, Clone: 252519D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87377-3-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02988-1

    Cytometric bead array sample test of MP02988-1

    The mean TRIM16 concentration was determined to be 1654.2 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load, 47.9 ng/mL in A431 cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP02988-1

    Cytometric bead array standard curve of MP02988-1

    Cytometric bead array standard curve of MP02988-1, TRIM16 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87377-3-PBS. Detection antibody: 87377-2-PBS. Standard: Ag21406. Range: 1.562-200 ng/mL.

TRIM16 Recombinant monoclonal antibody, PBS Only (Capture)

TRIM16 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 87377-3-PBS

产品说明书

CloneNo. 252519D5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

E3 ubiquitin-protein ligase TRIM16, EBBP, EC:2.3.2.27, Estrogen-responsive B box protein, tripartite motif containing 16

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TRIM16 Ready-To-Use IHC Kit. TRIM16 primary antibody included.

产品信息

87377-3-PBS targets TRIM16 as part of a matched antibody pair:

MP02988-1: 87377-3-PBS capture and 87377-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag21406

Product name: Recombinant human TRIM16 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-349 aa of BC015674

Sequence: EMQFGELLAAVRKAQANVMLFLEEKEQAALSQANGIKAHLEYRSAEMEKSKQELERMAAISNTVQFLEEYCKFKNTEDITFPSVYVGLKDKLSGIRKVITESTVHLIQLLENYKKKLQEFSKEEEYDIRTQVSAVVQRKYWTSKPEPSTREQFLQYAYDITFDPDTAHKYLRLQEENRKVTNTTPWEHPYPDLPSRFLHWRQVLSQQSLYLHRYYFEVEIFGAGTYVGLTCKGIDRKGEERNSCISGNNFSWSLQWNGKEFTAWYSDMETPLKAGPFRRLGVYIDFPGGILSFYGVEYDTMTLVHKFACKFSEPVYAAFWLSKKENAIRIVDLGEEPEKPAPSLVGTAP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tripartite motif-containing 16
别名 E3 ubiquitin-protein ligase TRIM16, EBBP, EC:2.3.2.27, Estrogen-responsive B box protein, tripartite motif containing 16
计算分子量 64 kDa
观测分子量 64 kDa
GenBank蛋白编号BC015674
基因名称 TRIM16
Gene ID (NCBI) 10626
RRIDAB_3745496
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO95361
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TRIM16 (tripartite motif-containing protein 16) is an E3 ubiquitin ligase belonging to the tripartite motif (TRIM) protein family, mediates the ubiquitination of its target proteins and tags them for degradation. TRIM16 controls ubiquitination of damaged compartments and regulates the core autophagy regulators ULK1, Beclin 1, and ATG16L1, which confer localized autophagic sequestration of damaged lysosomes (PMID: 27693506, 22629402). TRIM16 overexpression also enhances retinoidinduced differentiation, reduces neuroblastoma cell growth, migration, and significantly reduces tumorigenicity in vivo (PMID: 36504902). Ramos is the negative control.

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