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验证数据展示

  • WB analysis using 87342-1-RR (same clone as 87342-1-PBS)

    WB analysis using 87342-1-RR (same clone as 87342-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87342-1-RR (IRF4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87342-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 87342-1-RR (same clone as 87342-1-PBS)

    IHC staining of human tonsillitis using 87342-1-RR (same clone as 87342-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87342-1-RR (IRF4 antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87342-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IRF4 antibody included
  • IHC staining of human tonsillitis using 87342-1-RR (same clone as 87342-1-PBS)

    IHC staining of human tonsillitis using 87342-1-RR (same clone as 87342-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87342-1-RR (IRF4 antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87342-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IRF4 antibody included
  • Cytometric bead array sample test of MP02975-1

    Cytometric bead array sample test of MP02975-1

    The mean IRF4 concentration was determined to be 58.7 ng/mL in Ramos cell extract based on a 2.3 mg/mL extract load, THP-1 cell extract were served as a negative control.

  • Cytometric bead array standard curve of MP02975-1

    Cytometric bead array standard curve of MP02975-1

    Cytometric bead array standard curve of MP02975-1, IRF4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87342-1-PBS. Detection antibody: 87342-2-PBS. Standard: Ag1762. Range: 0.625-80 ng/mL.

  • Affinity of 87342-1-RR

    Affinity of 87342-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87342-1-RR against Human IRF4 were performed. The affinity constant is 2.43 nM.

IRF4 Recombinant monoclonal antibody, PBS Only (Capture)

IRF4 Uni-rAb® Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 87342-1-PBS

产品说明书

CloneNo. 252430B2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

MUM1/IRF4, interferon regulatory factor 4, IRF 4, IRF-4, LSIRF

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy IRF4 Ready-To-Use IHC Kit. IRF4 primary antibody included.

产品信息

87342-1-PBS targets IRF4 as part of a matched antibody pair:

MP02975-1: 87342-1-PBS capture and 87342-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1762

Product name: Recombinant human IRF4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-451 aa of BC015752

Sequence: MNLEGGGRGGEFGMSAVSCGNGKLRQWLIDQIDSGKYPGLVWENEEKSIFRIPWKHAGKQDYNREEDAALFKAWALFKGKFREGIDKPDPPTWKTRLRCALNKSNDFEELVERSQLDISDPYKVYRIVPEGAKKGAKQLTLEDPQMSMSHPYTMTTPYPSLPAQQVHNYMMPPLDRSWRDYVPDQPHPEIPYQCPMTFGPRGHHWQGPACENGCQVTGTFYACAPPESQAPGVPTEPSIRSAEALAFSDCRLHICLYYREILVKELTTSSPEGCRISHGHTYDASNLDQVLFPYPEDNGQRKNIEKLLSHLERGVVLWMAPDGLYAKRLCQSRIYWDGPLALCNDRPNKLERDQTCKLFDTQQFLSELQAFAHHGRSLPRFQVTLCFGEEFPDPQRQRKLITAHVEPLLARQLYYFAQQNSGHFLRGYDLPEHISNPEDYHRSIRHSSIQE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 IRF 4
别名 MUM1/IRF4, interferon regulatory factor 4, IRF 4, IRF-4, LSIRF
计算分子量 52 kDa
观测分子量 50-52 kDa
GenBank蛋白编号BC015752
基因名称 IRF4
Gene ID (NCBI) 3662
ENSEMBL Gene IDENSG00000137265
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ15306
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IRF4 is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the ISRE of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.

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