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验证数据展示

  • WB analysis using 87342-1-RR

    WB analysis using 87342-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87342-1-RR (IRF4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 87342-1-RR

    IHC staining of human tonsillitis using 87342-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87342-1-RR (IRF4 antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IRF4 antibody included
  • IHC staining of human tonsillitis using 87342-1-RR

    IHC staining of human tonsillitis using 87342-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87342-1-RR (IRF4 antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IRF4 antibody included
  • Affinity of 87342-1-RR

    Affinity of 87342-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87342-1-RR against Human IRF4 were performed. The affinity constant is 2.43 nM.

IRF4 Recombinant monoclonal antibody

IRF4 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 87342-1-RR

产品说明书

CloneNo. 252430B2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

MUM1/IRF4, interferon regulatory factor 4, IRF 4, IRF-4, LSIRF

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMJ cells, U266 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy IRF4 Ready-To-Use IHC Kit. IRF4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87342-1-RR targets IRF4 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1762

Product name: Recombinant human IRF4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-451 aa of BC015752

Sequence: MNLEGGGRGGEFGMSAVSCGNGKLRQWLIDQIDSGKYPGLVWENEEKSIFRIPWKHAGKQDYNREEDAALFKAWALFKGKFREGIDKPDPPTWKTRLRCALNKSNDFEELVERSQLDISDPYKVYRIVPEGAKKGAKQLTLEDPQMSMSHPYTMTTPYPSLPAQQVHNYMMPPLDRSWRDYVPDQPHPEIPYQCPMTFGPRGHHWQGPACENGCQVTGTFYACAPPESQAPGVPTEPSIRSAEALAFSDCRLHICLYYREILVKELTTSSPEGCRISHGHTYDASNLDQVLFPYPEDNGQRKNIEKLLSHLERGVVLWMAPDGLYAKRLCQSRIYWDGPLALCNDRPNKLERDQTCKLFDTQQFLSELQAFAHHGRSLPRFQVTLCFGEEFPDPQRQRKLITAHVEPLLARQLYYFAQQNSGHFLRGYDLPEHISNPEDYHRSIRHSSIQE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 IRF 4
别名 MUM1/IRF4, interferon regulatory factor 4, IRF 4, IRF-4, LSIRF
计算分子量 52 kDa
观测分子量 50-52 kDa
GenBank蛋白编号BC015752
基因名称 IRF4
Gene ID (NCBI) 3662
ENSEMBL Gene IDENSG00000137265
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ15306
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IRF4 is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the ISRE of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.

实验方案

Product Specific Protocols
IHC protocol for IRF4 antibody 87342-1-RRDownload protocol
WB protocol for IRF4 antibody 87342-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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