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验证数据展示

  • WB analysis using 87333-1-RR

    WB analysis using 87333-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87333-1-RR (TMEM201 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 87333-1-RR

    IF Staining of U2OS using 87333-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TMEM201 antibody (87333-1-RR, Clone: 252547D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HeLa using 87333-1-RR

    IF Staining of HeLa using 87333-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMEM201 antibody (87333-1-RR, Clone: 252547D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

TMEM201 Recombinant monoclonal antibody

TMEM201 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87333-1-RR

产品说明书

CloneNo. 252547D10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

NET5, SAMP1, Spindle-associated membrane protein 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHUVEC cells, mouse brain tissue, U2OS cells, mouse heart tissue
Positive IF/ICC detected inU2OS cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87333-1-RR targets TMEM201 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag21360

Product name: Recombinant human TMEM201 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 22-218 aa of BC137291

Sequence: VTACAAAGVLLYRIARRMKPTHTMVNCWFCNQDTLVPYGNRNCWDCPHCEQYNGFQENGDYNKPIPAQYLEHLNHVVSSAPSLRDPSQPQQWVSSQVLLCKRCNHHQTTKIKQLAAFAPREEGRYDEEVEVYRHHLEQMYKLCRPCQAAVEYYIKHQNRQLRALLLSHQFKRREADQTHAQNFSSAVKSPVQVILLR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transmembrane protein 201
别名 NET5, SAMP1, Spindle-associated membrane protein 1
计算分子量 666 aa, 72 kDa
观测分子量43 kDa and 72 kDa
GenBank蛋白编号BC137291
基因名称 TMEM201
Gene ID (NCBI) 199953
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ5SNT2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM201, also known as SAMP1 and NET5, is a member of the TMEM201 family. TMEM201 is a nuclear envelope transmembrane protein primarily localized to the inner nuclear membrane. It plays a crucial role in nuclear envelope architecture and is a component of the linker of nucleoskeleton and cytoskeleton (LINC) complex. TMEM201 is essential for proper nuclear division and nuclear membrane reorganization during mitosis (PMID: 35311970, PMID: 35311970). TMEM201 has two isoforms with the molecular weights of 43 kDa and 72 kDa, respectively.

实验方案

Product Specific Protocols
IF protocol for TMEM201 antibody 87333-1-RRDownload protocol
WB protocol for TMEM201 antibody 87333-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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