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验证数据展示

  • WB analysis using 87310-1-RR (same clone as 87310-1-PBS)

    WB analysis using 87310-1-RR (same clone as 87310-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87310-1-RR (CD316/IGSF8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87310-1-PBS in a different storage buffer formulation.

  • IF Staining of Neuro-2a using 87310-1-RR (same clone as 87310-1-PBS)

    IF Staining of Neuro-2a using 87310-1-RR (same clone as 87310-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using CD316/IGSF8 antibody (87310-1-RR, Clone: 252500B3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87310-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02958-1

    Cytometric bead array sample test of MP02958-1

    The mean IGSF8/CD316 concentration was determined to be 865.1 ng/mL in mouse brain tissue extract based on a 2.2 mg/mL extract load, A431 cell extract were served as a negative control.

  • Cytometric bead array standard curve of MP02958-1

    Cytometric bead array standard curve of MP02958-1

    Cytometric bead array standard curve of MP02958-1, MOUSE IGSF8/CD316 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87310-1-PBS. Detection antibody: 87310-2-PBS. Standard: Eg6320. Range: 0.625-80 ng/mL.

  • Affinity of 87310-1-RR

    Affinity of 87310-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87310-1-RR against Mouse CD316/IGSF8 were performed. The affinity constant is 0.658 nM.

Mouse CD316/IGSF8 Recombinant monoclonal antibody, PBS Only (Capture)

CD316/IGSF8 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 87310-1-PBS

产品说明书

CloneNo. 252500B3

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

CD316, CD81 partner 3, Ewi2, EWI-2, Glu-Trp-Ile EWI motif-containing protein 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87310-1-PBS targets CD316/IGSF8 as part of a matched antibody pair:

MP02958-1: 87310-1-PBS capture and 87310-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg6320

Product name: Recombinant mouse CD316/IGSF8 protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 26-577 aa of AF439263.1

Sequence: RQVHVPRGPLYRVAGTAVSISCNVSDYEGPAQQDFEWFMYRPEAPATSLGIVSTKDSQFSYAVFGPRVASGDLQVQRLKGDSVVLKIARLQAQDSGFYECYTPSTDTQYLGNYSAKVELRVLPDELQVSAAPPGPRGRQAATSPSRLTVHEGQELALGCLAQTKTKKHTHLSVSFGRAIPEAPVGRATLQEVVGLRSDMAVEAGAPYAERLASGELRLSKEGTDRYRMVVGGAQAGDSGTYHCTAAEWIQDPDGSWVQVAEKRAVLAHVDVQTLSSQLAVTVGPGERRIGPGEPLELLCNVSGALPPPGRHAAYSVGWEMAPAGAPGPGRLVAQLDTEGIGSLGPGYEDRHIAMEKVASRTYRLRLEAARPADAGTYRCLAKAYVRGSGTRLREAASARSRPLPVHVREEGVVLEAVAWLAGGTVYRGETASLLCNISVRGGPPGLRLAASWWVERPEEGELSSGPAQLVGGVGQDGVAELGVRPGGGPVSVELVGPRSHRLRLHGLGPEDEGIYHCAPSAWVQHADYSWYQAGSARSGPVTVYPYTHAVDT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 immunoglobulin superfamily, member 8
别名 CD316, CD81 partner 3, Ewi2, EWI-2, Glu-Trp-Ile EWI motif-containing protein 2
计算分子量65 kDa
观测分子量 70 kDa
GenBank蛋白编号AF439263.1
基因名称 Igsf8
Gene ID (NCBI) 140559
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8R366
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IGSF8 is also known as CD316, PGRL (PG regulatory-like protein), KAI/CD82-associated surface molecule, and CD81-binding partner 3 (CD81P3). IGSF8 contains four immunoglobulin domains, a transmembrane region, and a short cytoplasmic tail that does not bear any signature motif for signal transduction. IGSF8 is abundantly expressed in olfactory sensory neurons (OSN) axons and their developing synapses. (PMID: 17785435, PMID: 22687584)

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