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验证数据展示

  • WB analysis using 87302-4-RR

    WB analysis using 87302-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87302-4-RR (ATP6V1B2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 87302-4-RR

    WB analysis of mouse brain using 87302-4-RR

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 87302-4-RR (ATP6V1B2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 87302-4-RR

    IF Staining of HeLa using 87302-4-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ATP6V1B2 antibody (87302-4-RR, Clone: 252572H5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 87302-4-RR

    Affinity of 87302-4-RR

    Biolayer interferometry (BLl) kinetic assays of 87302-4-RR against Human ATP6V1B2 were performed. The affinity constant is below 1 pM.

ATP6V1B2 Recombinant monoclonal antibody

ATP6V1B2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87302-4-RR

产品说明书

CloneNo. 252572H5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

ATP6B1B2, ATP6B2, HO57, VATB, V-ATPase subunit B 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells, A549 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87302-4-RR targets ATP6V1B2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7174

Product name: Recombinant human ATP6V1B2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 162-511 aa of BC003100

Sequence: CRIYPEEMIQTGISAIDGMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKDVVDYSEENFAIVFAAMGVNMETARFFKSDFEENGSMDNVCLFLNLANDPTIERIITPRLALTTAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHADVSNQLYACYAIGKDVQAMKAVVGEEALTSDDLLYLEFLQKFERNFIAQGPYENRTVFETLDIGWQLLRIFPKEMLKRIPQSTLSEFYPRDSAKH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
别名 ATP6B1B2, ATP6B2, HO57, VATB, V-ATPase subunit B 2
计算分子量 57 kDa
观测分子量 56-58 kDa
GenBank蛋白编号BC003100
基因名称 ATP6V1B2
Gene ID (NCBI) 526
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP21281
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP6V1B2(V-type proton ATPase subunit B, brain isoform) is also named as ATP6B2, VPP3,HO57. It belongs to the ATPase alpha/beta chains family.V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.The quasi-ubiquitous ATP6V1B2 is one of 2 isoforms of ATP6V1 and is expressed in most cell types, where it plays a major role in organelle acidification (PMID:18667600).

实验方案

Product Specific Protocols
IF protocol for ATP6V1B2 antibody 87302-4-RRDownload protocol
WB protocol for ATP6V1B2 antibody 87302-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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