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验证数据展示

  • WB analysis of MCF-7 using 87176-3-RR

    WB analysis of MCF-7 using 87176-3-RR

    WB result of FAM84B antibody (87176-3-RR; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAM84B transfected MCF-7 cells.

  • WB analysis using 87176-3-RR

    WB analysis using 87176-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87176-3-RR (FAM84B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 87176-3-RR

    WB analysis using 87176-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87176-3-RR (FAM84B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 87176-3-RR

    IF Staining of MCF-7 using 87176-3-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using FAM84B antibody (87176-3-RR, Clone: 252473G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 87176-3-RR

    Affinity of 87176-3-RR

    Biolayer interferometry (BLl) kinetic assays of 87176-3-RR against Human FAM84B were performed. The affinity constant is 4.50 nM.

  • KD/KO Validated

FAM84B Recombinant monoclonal antibody

FAM84B Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87176-3-RR

产品说明书

CloneNo. 252473G6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

LRAT domain-containing 2, LRATD2, NSE2, Protein FAM84B, Protein LRATD2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, mouse stomach tissue, MDA-MB-453 cells, rat stomach tissue
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87176-3-RR targets FAM84B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13243

Product name: Recombinant human FAM84B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-310 aa of BC052957

Sequence: MGNQVEKLTHLSYKEVPTADPTGVDRDDGPRIGVSYIFSNDDEDVEPQPPPQGPDGGGLPDGGDGPPPPQPQPYDPRLHEVECSVFYRDECIYQKSFAPGSAALSTYTPENLLNKCKPGDLVEFVSQAQYPHWAVYVGNFQVVHLHRLEVINSFLTDASQGRRGRVVNDLYRYKPLSSSAVVRNALAHVGAKERELSWRNSESFAAWCRYGKREFKIGGELRIGKQPYRLQIQLSAQRSHTLEFQSLEDLIMEKRRNDQIGRAAVLQELATHLHPAEPEEGDSNVARTTPPPGRPPAPSSEEEDGEAVAH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 family with sequence similarity 84, member B
别名 LRAT domain-containing 2, LRATD2, NSE2, Protein FAM84B, Protein LRATD2
计算分子量 34 kDa
观测分子量40 kDa
GenBank蛋白编号BC052957
基因名称 FAM84B
Gene ID (NCBI) 157638
RRIDAB_3745391
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96KN1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM84B (Family with sequence similarity 84 member B) is a protein-coding gene located on human chromosome 8. Although its full physiological function remains under investigation, accumulating evidence links FAM84B to cell proliferation, cytoskeletal organization and cancer progression. In several tumor types-most notably prostate, esophageal squamous-cell, breast and glioma-FAM84B is frequently amplified or over-expressed, and high expression often correlates with advanced stage, higher Gleason score and poorer patient survival.

实验方案

Product Specific Protocols
IF protocol for FAM84B antibody 87176-3-RRDownload protocol
WB protocol for FAM84B antibody 87176-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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