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验证数据展示

  • WB analysis using 87171-1-RR (same clone as 87171-1-PBS)

    WB analysis using 87171-1-RR (same clone as 87171-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87171-1-RR (COIL antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87171-1-PBS in a different storage buffer formulation.

  • WB analysis using 87171-1-RR (same clone as 87171-1-PBS)

    WB analysis using 87171-1-RR (same clone as 87171-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87171-1-RR (COIL antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87171-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 87171-1-RR (same clone as 87171-1-PBS)

    WB analysis of HEK-293 using 87171-1-RR (same clone as 87171-1-PBS)

    WB result of Coilin antibody (87171-1-RR; 1:300000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Coilin transfected HEK-293 cells. This data was developed using the same antibody clone with 87171-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02891-1

    Sandwich ELISA sample test of MP02891-1

    The mean Coilin concentration was determined to be 19.9 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load and 9.6 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02891-1

    Sandwich ELISA standard curve of MP02891-1

    Sandwich ELISA standard curve of MP02891-1, Human Coilin Recombinant Matched Antibody Pair - PBS only. 87171-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1416. 87171-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • IF Staining of HepG2 using 87171-1-RR (same clone as 87171-1-PBS)

    IF Staining of HepG2 using 87171-1-RR (same clone as 87171-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COIL antibody (87171-1-RR, Clone: 252327B11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87171-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02891-1

    Cytometric bead array sample test of MP02891-1

    The mean COIL/Coilin concentration was determined to be 63.3 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load, 29.8 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load, 88.3 ng/mL in K-562 cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP02891-1

    Cytometric bead array standard curve of MP02891-1

    Cytometric bead array standard curve of MP02891-1, COIL/Coilin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87171-2-PBS. Detection antibody: 87171-1-PBS. Standard: Ag1416. Range: 0.312-40 ng/mL.

  • Affinity of 87171-1-RR

    Affinity of 87171-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87171-1-RR against Human Coilin were performed. The affinity constant is below 1 pM.

Coilin Recombinant monoclonal antibody, PBS Only (Detector)

Coilin Uni-rAb® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 87171-1-PBS

产品说明书

CloneNo. 252327B11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

别名

COIL, CLN80, p80 coilin, p80-coilin

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87171-1-PBS targets Coilin as part of a matched antibody pair:

MP02891-1: 87171-2-PBS capture and 87171-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1416

Product name: Recombinant human COIL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 212-576 aa of BC010385

Sequence: ANQRCSSPKGSARNSLVKAKRKGSVSVCSKESPSSSSESESCDESISDGPSKVTLEARNSSEKLPTELSKEEPSTKNTTADKLAIKLGFSLTPSKGKTSGTTSSSSDSSAESDDQCLMSSSTPECAAGFLKTVGLFAGRGRPGPGLSSQTAGAAGWRRSGSNGGGQAPGASPSVSLPASLGRGWGREENLFSWKGAKGRGMRGRGRGRGHPVSCVVNRSTDNQRQQQLNDVVKNSSTIIQNPVETPKKDYSLLPLLAAAPQVGEKIAFKLLELTSSYSPDVSDYKEGRILSHNPETQQVDIEILSSLPALREPGKFDLVYHNENGAEVVEYAVTQESKITVFWKELIDPRLIIESPSNTSSTEPA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 coilin
别名 COIL, CLN80, p80 coilin, p80-coilin
计算分子量 576 aa, 63 kDa
观测分子量75 kDa
GenBank蛋白编号BC010385
基因名称 COIL
Gene ID (NCBI) 8161
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP38432
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The nuclear coiled body(CBs) and nuclear structures known as gems contain high concentrations of the survival motor neurons (SMN) protein complex. CBs and gems often colocalize, and communication between them is mediated by COIL. COIL is a component of the CBS which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBs disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin, and symmetrical dimethylation of the COIL RG box motif is a molecular switch that determines whether the SMN complex forms gems or becomes incorporated into CBs.

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