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验证数据展示

  • WB analysis of HT-29 using 87107-2-RR (same clone as 87107-2-PBS)

    WB analysis of HT-29 using 87107-2-RR (same clone as 87107-2-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 87107-2-RR (MUC13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87107-2-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 87107-2-RR (same clone as 87107-2-PBS)

    IHC staining of human colon using 87107-2-RR (same clone as 87107-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 87107-2-RR (MUC13 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87107-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MUC13 antibody included
  • IHC staining of human colon using 87107-2-RR (same clone as 87107-2-PBS)

    IHC staining of human colon using 87107-2-RR (same clone as 87107-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 87107-2-RR (MUC13 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87107-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MUC13 antibody included
  • IF Staining of HT-29 using 87107-2-RR (same clone as 87107-2-PBS)

    IF Staining of HT-29 using 87107-2-RR (same clone as 87107-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using MUC13 antibody (87107-2-RR, Clone: 252211G3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87107-2-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02867-1

    Cytometric bead array sample test of MP02867-1

    The mean MUC13 concentration was determined to be 10.6 ng/mL in SW480 cell extract based on a 1.2 mg/mL extract load. A431 cell extract were served as a negative control.

  • Cytometric bead array standard curve of MP02867-1

    Cytometric bead array standard curve of MP02867-1

    Cytometric bead array standard curve of MP02867-1, MUC13 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87107-2-PBS. Detection antibody: 87107-1-PBS. Standard: Eg6586. Range: 0.625-80 ng/mL.

  • Affinity of 87107-2-RR

    Affinity of 87107-2-RR

    Biolayer interferometry (BLl) kinetic assays of 87107-1-RR against Human MUC13 were performed. The affinity constant is below 1 pM.

MUC13 Recombinant monoclonal antibody, PBS Only (Capture)

MUC13 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 87107-2-PBS

产品说明书

CloneNo. 252211G3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

Down-regulated in colon cancer 1, DRCC1, MUC-13, Mucin-13, RECC

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MUC13 Ready-To-Use IHC Kit. MUC13 primary antibody included.

产品信息

87107-2-PBS targets MUC13 as part of a matched antibody pair:

MP02867-1: 87107-2-PBS capture and 87107-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg6586

Product name: Recombinant Human MUC13 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 19-421 aa of NM_033049.4

Sequence: TNQGNSADAVTTTETATSGPTVAAADTTETNFPETASTTANTPSFPTATSPAPPIISTHSSSTIPTPAPPIISTHSSSTIPIPTAADSESTTNVNSLATSDIITASSPNDGLITMVPSETQSNNEMSPTTEDNQSSGPPTGTALLETSTLNSTGPSNPCQDDPCADNSLCVKLHNTSFCLCLEGYYYNSSTCKKGKVFPGKISVTVSETFDPEEKHSMAYQDLHSEITSLFKDVFGTSVYGQTVILTVSTSLSPRSEMRADDKFVNVTIVTILAETTSDNEKTVTEKINKAIRSSSSNFLNYDLTLRCDYYGCNQTADDCLNGLACDCKSDLQRPNPQSPFCVASSLKCPDACNAQHKQCLIKKSGGAPECACVPGYQEDANGNCQKCAFGYSGLDCKDKFQL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mucin 13, cell surface associated
别名 Down-regulated in colon cancer 1, DRCC1, MUC-13, Mucin-13, RECC
计算分子量55 kDa
观测分子量120 kDa
GenBank蛋白编号NM_033049.4
基因名称 MUC13
Gene ID (NCBI) 56667
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9H3R2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mucins (MUC) are highly glycosylated proteins that are abundantly expressed in mammalian epithelial cells. Mucin 13 (MUC13) is a member of the transmembrane glycoprotein mucins, which are normally expressed in the large intestine, trachea, kidney, small intestine, and gastric epithelium (PMID: 22027689). MUC13 is involved in the tumorigenesis of multiple malignancies, including pancreatic cancer, colorectal cancer, renal cancer, and lung cancer (PMID: 22027689; PMID: 31427737; PMID: 28205224; PMID: 34966453).

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