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验证数据展示

  • WB analysis using 87051-3-RR

    WB analysis using 87051-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87051-3-RR (SOX10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A375 using 87051-3-RR

    IF Staining of A375 using 87051-3-RR

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using SOX10 antibody (87051-3-RR, Clone: 252024E10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 87051-3-RR

    Affinity and Kinetic Characterization of 87051-3-RR

    Biolayer interferometry (BLl) kinetic assays of 87051-3-RR against Human SOX10 were performed. The affinity constant is 66.0 pM.

SOX10 Recombinant monoclonal antibody

SOX10 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87051-3-RR

产品说明书

CloneNo. 252024E10

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, ELISA

别名

DOM, PCWH, SRY-box transcription factor 9, Transcription factor SOX 10, Transcription factor SOX-10

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMDA-MB-453 cells, A375 cells, SW480 cells, C6 cells
Positive IF/ICC detected inA375 cells
Planning an IHC experiment? We recommend our IHCeasy SOX10 Ready-To-Use IHC Kit. SOX10 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87051-3-RR targets SOX10 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag38090

Product name: Recombinant human SOX10 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 267-466 aa of BC002824

Sequence: GKPHIDFGNVDIGEISHEVMSNMETFDVAELDQYLPPNGHPGHVSSYSAAGYGLGSALAVASGHSAWISKPPGVALPTVSPPGVDAKAQVKTETAGPQGPPHYTDQPSTSQIAYTSLSLPHYGSAFPSISRPQFDYSDHQPSGPYYGHSGQASGLYSAFSYMGPSQRPLYTAISDPSPSGPQSHSPTHWEQPVYTTLSRP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SRY (sex determining region Y)-box 10
别名 DOM, PCWH, SRY-box transcription factor 9, Transcription factor SOX 10, Transcription factor SOX-10
计算分子量 50 kDa
观测分子量50-65 kDa
GenBank蛋白编号BC002824
基因名称 SOX10
Gene ID (NCBI) 6663
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP56693
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sox10 is a transcription factor that has a crucial role in complex signalling pathways regulating central nervous system and neural crest development. The calcualted molecular weight of SOX10 is 50 kDa, but modified SOX10 is about 55-65 kDa. (PMID: 23799842, 39982575)

实验方案

Product Specific Protocols
IF protocol for SOX10 antibody 87051-3-RRDownload protocol
WB protocol for SOX10 antibody 87051-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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