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验证数据展示

  • WB analysis using 87051-3-RR (same clone as 87051-3-PBS)

    WB analysis using 87051-3-RR (same clone as 87051-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87051-3-RR (SOX10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87051-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02850-2

    Sandwich ELISA sample test of MP02850-2

    The mean SOX10 concentration was determined to be 24.4 ng/mL in MDA-MB-453 cell extract based on a 1.5 mg/mL extract load and 0.2 ng/mL in A431 cell extract based on a 1.2 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02850-2

    Sandwich ELISA standard curve of MP02850-2

    Sandwich ELISA standard curve of MP02850-2, Human SOX10 Recombinant Matched Antibody Pair - PBS only. 87051-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag38090. 87051-4-PBS was HRP conjugated as the detection antibody. Range: 31.3-1000 pg/mL.

  • IF Staining of A375 using 87051-3-RR (same clone as 87051-3-PBS)

    IF Staining of A375 using 87051-3-RR (same clone as 87051-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using SOX10 antibody (87051-3-RR, Clone: 252024E10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87051-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 87051-3-RR

    Affinity and Kinetic Characterization of 87051-3-RR

    Biolayer interferometry (BLl) kinetic assays of 87051-3-RR against Human SOX10 were performed. The affinity constant is 66.0 pM.

SOX10 Recombinant monoclonal antibody, PBS Only (Capture)

SOX10 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA
Cat No. 87051-3-PBS

产品说明书

CloneNo. 252024E10

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, Sandwich ELISA, Indirect ELISA

别名

DOM, PCWH, SRY-box transcription factor 9, Transcription factor SOX 10, Transcription factor SOX-10

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SOX10 Ready-To-Use IHC Kit. SOX10 primary antibody included.

产品信息

87051-3-PBS targets SOX10 as part of a matched antibody pair:

MP02850-2: 87051-3-PBS capture and 87051-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag38090

Product name: Recombinant human SOX10 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 267-466 aa of BC002824

Sequence: GKPHIDFGNVDIGEISHEVMSNMETFDVAELDQYLPPNGHPGHVSSYSAAGYGLGSALAVASGHSAWISKPPGVALPTVSPPGVDAKAQVKTETAGPQGPPHYTDQPSTSQIAYTSLSLPHYGSAFPSISRPQFDYSDHQPSGPYYGHSGQASGLYSAFSYMGPSQRPLYTAISDPSPSGPQSHSPTHWEQPVYTTLSRP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SRY (sex determining region Y)-box 10
别名 DOM, PCWH, SRY-box transcription factor 9, Transcription factor SOX 10, Transcription factor SOX-10
计算分子量 50 kDa
观测分子量50-65 kDa
GenBank蛋白编号BC002824
基因名称 SOX10
Gene ID (NCBI) 6663
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP56693
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Sox10 is a transcription factor that has a crucial role in complex signalling pathways regulating central nervous system and neural crest development. The calcualted molecular weight of SOX10 is 50 kDa, but modified SOX10 is about 55-65 kDa. (PMID: 23799842, 39982575)

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