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验证数据展示

  • WB analysis using 86960-1-RR (same clone as 86960-1-PBS)

    WB analysis using 86960-1-RR (same clone as 86960-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86960-1-RR (JAM3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86960-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 86960-1-RR (same clone as 86960-1-PBS)

    IF Staining of U-251 using 86960-1-RR (same clone as 86960-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using JAM3 antibody (86960-1-RR, Clone: 251954C5) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86960-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02796-1

    Cytometric bead array sample test of MP02796-1

    The mean JAM3 concentration was determined to be 23.0 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load, 30.4 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load, HeLa cell extract were served as a negative control.

  • Cytometric bead array standard curve of MP02796-1

    Cytometric bead array standard curve of MP02796-1

    Cytometric bead array standard curve of MP02796-1, JAM3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86960-2-PBS. Detection antibody: 86960-1-PBS. Standard: Eg6611. Range: 0.156-20 ng/mL.

  • Affinity and Kinetic Characterization of 86960-1-RR

    Affinity and Kinetic Characterization of 86960-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86960-1-RR against Human JAM3 were performed. The affinity constant is 0.527 nM.

JAM3 Recombinant monoclonal antibody, PBS Only (Detector)

JAM3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 86960-1-PBS

产品说明书

CloneNo. 251954C5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

JAM-2, JAM-3, JAM-C, Junctional adhesion molecule 3, Junctional adhesion molecule C

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86960-1-PBS targets JAM3 as part of a matched antibody pair:

MP02796-1: 86960-2-PBS capture and 86960-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Eg6611

Product name: Recombinant Human JAM3 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 32-241 aa of NM_032801.5

Sequence: VNLKSSNRTPVVQEFESVELSCIITDSQTSDPRIEWKKIQDEQTTYVFFDNKIQGDLAGRAEILGKTSLKIWNVTRRDSALYRCEVVARNDRKEIDEIVIELTVQVKPVTPVCRVPKAVPVGKMATLHCQESEGHPRPHYSWYRNDVPLPTDSRANPRFRNSSFHLNSETGTLVFTAVHKDDSGQYYCIASNDAGSARCEEQEMEVYDLN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 junctional adhesion molecule 3
别名 JAM-2, JAM-3, JAM-C, Junctional adhesion molecule 3, Junctional adhesion molecule C
计算分子量35 kDa
观测分子量40 kDa
GenBank蛋白编号NM_032801.5
基因名称 JAM3
Gene ID (NCBI) 83700
RRIDAB_3745236
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9BX67-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Junctional adhesion molecule 3 (JAM3) is a type I transmembrane glycoprotein containing two Ig-like domains, which is expressed in various tissues and plays a crucial role in cell junctions, cell polarity, and motility (PMID: 34292449; 28931565). It has been proposed that JAM3 participates in leukocyte-platelet interactions, as well as angiogenesis and brain development (PMID: 12208882; 23255084). JAM3 may also be a new marker for predicting the prognosis and immune functions of bladder cancer patients (PMID: 38400838).

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