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验证数据展示

  • WB analysis using 86960-1-RR

    WB analysis using 86960-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86960-1-RR (JAM3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of U-251 using 86960-1-RR

    IF Staining of U-251 using 86960-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using JAM3 antibody (86960-1-RR, Clone: 251954C5) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 86960-1-RR

    Affinity and Kinetic Characterization of 86960-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86960-1-RR against Human JAM3 were performed. The affinity constant is 0.527 nM.

JAM3 Recombinant monoclonal antibody

JAM3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86960-1-RR

产品说明书

CloneNo. 251954C5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

JAM-2, JAM-3, JAM-C, Junctional adhesion molecule 3, Junctional adhesion molecule C

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSH-SY5Y cells, SK-N-SH cells, A375 cells, Jurkat cells, HEK-293 cells, human heart tissue, mouse heart tissue
Positive IF/ICC detected inU-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86960-1-RR targets JAM3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Eg6611

Product name: Recombinant Human JAM3 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 32-241 aa of NM_032801.5

Sequence: VNLKSSNRTPVVQEFESVELSCIITDSQTSDPRIEWKKIQDEQTTYVFFDNKIQGDLAGRAEILGKTSLKIWNVTRRDSALYRCEVVARNDRKEIDEIVIELTVQVKPVTPVCRVPKAVPVGKMATLHCQESEGHPRPHYSWYRNDVPLPTDSRANPRFRNSSFHLNSETGTLVFTAVHKDDSGQYYCIASNDAGSARCEEQEMEVYDLN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 junctional adhesion molecule 3
别名 JAM-2, JAM-3, JAM-C, Junctional adhesion molecule 3, Junctional adhesion molecule C
计算分子量35 kDa
观测分子量40 kDa
GenBank蛋白编号NM_032801.5
基因名称 JAM3
Gene ID (NCBI) 83700
RRIDAB_3745236
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9BX67-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Junctional adhesion molecule 3 (JAM3) is a type I transmembrane glycoprotein containing two Ig-like domains, which is expressed in various tissues and plays a crucial role in cell junctions, cell polarity, and motility (PMID: 34292449; 28931565). It has been proposed that JAM3 participates in leukocyte-platelet interactions, as well as angiogenesis and brain development (PMID: 12208882; 23255084). JAM3 may also be a new marker for predicting the prognosis and immune functions of bladder cancer patients (PMID: 38400838).

实验方案

Product Specific Protocols
IF protocol for JAM3 antibody 86960-1-RRDownload protocol
WB protocol for JAM3 antibody 86960-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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